Human antibodies to respiratory syncytial virus F protein and methods of use thereof
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsAnne Gurnett-Bander, David Perez-Caballero, Sumathi Sivapalasingam, Xunbao Duan, Douglas MacDonald
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Abstract
The present invention provides fully human antibodies that bind to respiratory syncytial virus F protein, compositions comprising the antibodies and methods of use. The antibodies of the invention are useful for preventing fusion of the virus with the cell membrane and preventing cell to cell spread of the virus, thereby providing a means of preventing the infection, or treating a patient suffering from the infection and ameliorating one or more symptoms or complications associated with the viral infection. The antibodies may also be useful for diagnosis of an infection by RSV.
Claims46
An isolated human antibody or antigen-binding fragment thereof that binds specifically to RSV-F, wherein the antibody or antigen-binding fragment comprises three heavy chain complementarity determining regions (CDRs) (HCDR1, HCDR2 and HCDR3) contained within any one of the heavy chain variable region (HCVR) amino acid sequences selected from the group consisting of SEQ ID NO:
- 2, 18, 34, 50, 66, 82, 98, 114, 130, 146, 162, 178, 194, 210, 226, 242, 258, 274, 290, 306, 322 and 338;
- and comprises three light chain CDRs (LCDR1, LCDR2 and LCDR3) contained within any one of the light chain variable region (LCVR) amino acid sequences selected from the group consisting of SEQ ID NOs:
- 10, 26, 42, 58, 74, 90, 106, 122, 138, 154, 170, 186, 202, 218, 234, 250, 266, 282, 298, 314, 330 and 346.
An isolated antibody or antigen-binding fragment thereof that competes for specific binding to RSV-F with an antibody or antigen-binding fragment comprising heavy and light chain sequence pairs selected from the group consisting of SEQ ID NOs:
- 2/10, 18/26, 34/42, 50/58, 66/74, 82/90, 98/106, 114/122, 130/138, 146/154, 162/170, 178/186, 194/202, 210/218, 226/234, 242/250, 258/266, 274/282, 290/298, 306/314, 322/330 and 338/346, wherein the antibody that competes for specific binding to RSV-F interacts with an epitope comprising an amino acid sequence ranging from about position 161 to about position 188 of SEQ ID NO:354, or interacts with the serine at position 173 of SEQ ID NO:
- 354, and/or the threonine at position 174 of SEQ ID NO:
- 354.
An isolated antibody or antigen-binding fragment thereof that binds the same epitope on RSV-F that is recognized by an antibody comprising heavy and light chain sequence pairs selected from the group consisting of SEQ ID NOs:
- 2/10, 18/26, 34/42, 50/58, 66/74, 82/90, 98/106, 114/122, 130/138, 146/154, 162/170, 178/186, 194/202, 210/218, 226/234, 242/250, 258/266, 274/282, 290/298, 306/314, 322/330 and 338/346, wherein the epitope comprises an amino acid sequence ranging from about position 161 to about position 188 of SEQ ID NO:354.
Dependencies are inferred from the claim text.