Polypeptides having alpha-glucuronidase activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsJohan Börjesson, Anders Viksø-Nielsen, Nikolaj Spodsberg, Kristian Krogh
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Abstract
The present invention relates to isolated polypeptides having alpha-glucuronidase activity, catalytic domains and polynucleotides encoding the polypeptides, catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains.
Claims29
A nucleic acid construct comprising a polynucleotide encoding a polypeptide having alpha-qlucuronidase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide having alpha-qlucuronidase activity is selected from the group consisting of:
- (a) a polypeptide having at least 80% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5× SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2× SSC, 0.2% SDS at 65° C.;
- and
- (c) a polypeptide encoded by a polynucleotide having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:1 or the cDNA sequence thereof.
Dependencies are inferred from the claim text.