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US 9,446,075 B2Granted
Compositions derived from stem cell released molecules and methods for formulation thereof
AssigneeBioregenerative Sciences
InventorGreg Maguire
Life of this patent
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Abstract
Compositions for use in treatment of a variety of tissue diseases include stem cells and stem cell released molecules (SRM's) suspended in an aqueous solution with a cellulosic material or other thickening agent. The stem cells and SRM's can be derived from one or more distinct cell lines. The SRM's can further include one or more mucins, cytokines, or growth factors. Exemplary formulations include stem cells and SRMs derived from epithelial stem cells, corneal limbal stem cells, and fibroblasts. Other compositions and methods for formulation thereof are described.
Claims14
A method for forming a therapeutic composition, comprising:
- culturing cells of a first cell line and a second cell line in a first and a second respective culture medium, wherein said cells secrete extracellular products into the respective culture medium so that a first conditioned medium and a second conditioned medium are respectively formed;
- harvesting said first and second conditioned medium by removing cultured cells from said respective conditioned medium;
- and
- combining said first conditioned medium and second conditioned medium to form a therapeutic composition,
- wherein said first and second cell lines are an adult human adipose-derived stem cell line (ADSC), and a fetal human fibroblast cell line.
2 more elements
Dependencies are inferred from the claim text.