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US 9,441,253 B2Granted
Metabolic transistor in bacteria
AssigneeWilliam Marsh Rice University
InventorsKa-Yiu San, George N. Bennett, Hui Wu
Life of this patent
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Abstract
The disclosure relates to a metabolic transistor in bacteria where a competitive pathway is introduced to compete with a product pathway for available carbon so as to control the carbon flux in the bacteria.
Claims7
A method of increasing the production of a product in a bacteria, said method comprising:
- a) providing a bacteria having a product pathway producing a product, wherein a competitive pathway competes with said product pathway for available carbon, said competitive pathway requiring a cofactor that is not rate limiting;
- b) introducing one or more diverting gene(s) under the control of a promoter into said bacteria to directly or indirectly divert said cofactor or an intermediate in the formation of the cofactor away from said competitive pathway;
- c) allowing expression of said diverting gene(s) and reducing levels of said cofactor, and thus reducing levels of said competitive pathway;
- d) thereby increasing said product pathway and said product;
- wherein said cofactor and said one or more diverting genes and said products are selected from the group consisting of:
- Cofactor:
- Diverting gene(s) encoding:
- Product:
- ubiquinone
- geranylgeranyl diphosphate
- Fermentative products or
- synthase and phytoene
- lactate or acetate ethanol or
- synthase and phytoene
- formate, or acetate or
- desaturase
- succinate or 2,3 butane diol
- ubiquinone
- geranyl diphosphate:
- 4-
- Fermentative products or
- hydroxybenzoate
- lactate or acetate ethanol or
- geranyltransferase
- formate, or acetate or
- succinate or 2,3 butane diol
- biotin
- Biotin-CoA ligase or biotin
- Glutamate or lysine
- CoA synthetase
- pantothenate
- pantothenate hydrolyase
- Valine
- thiamine
- thiamine pyridinylase or
- Fermentative products or
- aminopyrimidine
- lactate or ethanol or formate,
- aminohydrolase
- or acetate or succinate or 2,3
- butane diol pr
- Folate or 4-
- 4-aminobenzoate 1-
- Serine
- amino-
- monooxygenase or
- benzoate
- aminobenzoate
- decarboxylase
- Heme
- Siroheme synthase
- Fermentative products or
- lactate or acetate ethanol or
- formate or acetate or succinate
- or 2,3 butane diol
- 3-Methyl-2-
- 2-oxoisovalerate
- PHE or TYR.
- oxobutanoic
- dehydrogenase or 3-methyl-
- acid
- 2-oxobutanoate
- dehydrogenase
60 more elements
Dependencies are inferred from the claim text.