Loading patent…
AssigneeE. I. Du Pont de Nemours and Company
InventorsQuinn Qun Zhu, Seung-Pyo Hong, Dongming Xie, Zhixiong Xue, Hyeryoung Yoon, Michael Dauner
Estimating the term…
Recombinant microbial cells are disclosed herein that comprise (i) a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein, and (ii) a polyunsaturated fatty acid (PUFA) biosynthetic pathway. The down-regulation of the polynucleotide sequence encoding Sou2 sorbitol utilization protein can increase the lipid content of the microbial cells and/or decrease the total amount of sugar alcohols produced by the microbial cells. Also disclosed are methods of using the recombinant microbial cells to produce oil containing omega-3 polyunsaturated fatty acids such as EPA.
A recombinant microbial cell comprising a down-regulation of an endogenous polynucleotide sequence encoding Sou2 sorbitol utilization protein selected from the group consisting of Aspergillus niger, Bifidobacterium dentium, Candida albicans, Candida dubliniensis, Candida orthopsilosis, Candida parapsilosis, Cordyceps militaris, Debaryomyces hansenii, Enterobacter hormaechei, Gaeumannomyces graminis, Glarea lozoyensis, Lodderomyces elongisporus, Magnaporthe oryzae, Metarhizium acridum, Metarhizium anisopliae, Mycobacterium smegmatis, Neurospora crassa, Neurospora tetrasperma, Paracoccidioides brasiliensis, Penicillium chrysogenum, Scheffersomyces stipitis, Uncinocarpus reesii, Verticillium dahliae, and Yarrowia lipolytica, wherein said down-regulation decreases the total amount of sugar alcohols produced by the microbial cell, as compared to a suitable control cell.
Dependencies are inferred from the claim text.