Cell culture apparatus, cell culture system and cell culture method

A cell culture method comprising:

• placing on an upper surface of a container table a culture container formed of a soft packing material;
• dividing an enclosure part of the culture container into two or more chambers by placing on an upper surface of the culture container a movable partition roller so as to form a culture part for enclosing a culture medium and/or cells being cultured;
• pressing the upper surface of the culture container with the partition roller such that a first inner surface of the culture container contacts a second inner surface of the culture container opposite the first inner surface so as to segregate the two or more chambers from each other;
• and
• changing a volume of the culture part by moving a rotation axis of the partition roller with the partition roller in a radial direction relative to the culture container, while allowing the partition roller to rotate and maintaining the partition roller to press the upper surface of the culture container such that the first inner surface of the culture container contacts the second inner surface of the culture container,
• wherein the two or more chambers comprise an extensible part, and the partition roller segregates the extensible part from the culture part so as to enclose an interior space of the culture part from the extensible part, and
• wherein a volume of an interior space of the culture part changes in a continuous manner while moving the partition roller and/or the culture container.