Multi-element isotopic measurement by direct coupling of a multi-cycle isotachophoresis technique and a mass spectrometry technique
AssigneeCommissariat A L'energie Atomique Et Aux Energies Alternatives
InventorsFrédéric Chartier, Laurent Vio, Anthony Nonell, Hélène Isnard
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Abstract
A method for separating electrically charged species contained in a solution by an isotachophoresis method applied in an electrophoresis device, the isotachophoresis method being coupled to isotopic measurement using a mass spectrometer. The method notably comprises a step of stopping the voltage applied to the terminal of the electrophoresis capillary and transient application of a counter-pressure making it possible to utilize the length of the capillary several times and extend the separation distance artificially.
Claims11
A method for separating electrically charged species contained in a solution by an isotachophoresis method comprising:
- a first step wherein the following are introduced into the inlet of an electrophoresis capillary, in this order:
- a leading electrolyte with a first speed of electrophoretic migration, a solution comprising charged species with their own individual speeds of electrophoretic migration and a terminating electrolyte with a second speed of electrophoretic migration, the first speed of electrophoretic migration being the largest of the speeds of migration and the second speed being the smallest of the speeds of migration;
- the following steps are repeated until separation of at least one charged species contained in the solution is obtained:
- a second step in which a voltage is applied between the two ends of the capillary so as to start separation of the species,
- a third step in which the voltage is stopped,
- a fourth step in which a counter-pressure is applied so as to displace the species towards the inlet of the capillary, the speed of displacement of the species during application of the counter-pressure being lower than the speed of electrophoretic migration of the species under the effect of the voltage.
Dependencies are inferred from the claim text.