# US 9,435,812 B2 · Expression of ETS related gene (ERG) and phosphatase and tensin homolog (PTEN) correlates with prostate cancer capsular penetration > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,435,812 B2 - Document ID: US9435812B2 - Status: Granted - Kind: B2 - Application number: 13/596,266 - Filed: 2012-08-28 - Published: 2013-08-01 - Granted: 2016-09-06 - Earliest claimed: 2011-08-31 - Assignees: Ventana Medical Systems, Inc.; Arizona Board of Regents on Behalf of the University of Arizona - Applicants: Gary Pestano; Ray B. Nagle; Connie Cortez; Kristie A. Vanpatten; Amit M. Algotar - Inventors: Gary Pestano; Ray B. Nagle; Connie Cortez; Kristie A. Vanpatten; Amit M. Algotar - Web page: https://patentgrep.com/patent/US9435812B2 ## CPC - C12Q 1/6886: for cancer (immunoassay for cancer G01N33/575) - G01N 33/57555: of the prostate - G01N 33/57575: {involving oncogenic proteins} - G01N 33/6893: {related to diseases not provided for elsewhere} - C12Q 2600/118: Prognosis of disease development - C12Q 2600/158: Expression markers - C12Q 2600/16: Primer sets for multiplex assays - G01N 2333/47: Assays involving proteins of known structure or function as defined in the subgroups - C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The disclosure provides methods for characterizing a prostate cancer sample by detecting expression of ERG, PTEN or both, changes in which relative to a normal control are shown herein to be correlated with prostate cancer capsular penetration and more aggressive forms of prostate cancer. Such methods are useful for the prognosis of prostate cancer capsular penetration and for making treatment decisions in patients with prostate cancer that has penetrated the capsule. Also provided are kits that can be used with such methods. ## Claims ### Claim 1 A method for determining risk that a prostate cancer will penetrate the prostatic capsule, comprising: contacting a prostate cancer sample isolated from a subject with an ETS related gene (ERG)-specific antibody and a phosphatase and tensin homolog (PTEN)-specific antibody; measuring increased protein expression of ERG in the prostate cancer sample relative to a control representing ERG protein expression expected in a normal prostate sample; measuring decreased PTEN in the prostate cancer sample relative to a control representing PTEN protein expression expected in a normal prostate sample; determining that there is a higher risk that the prostate cancer has penetrated or will likely penetrate the prostatic capsule based on the ERG measuring and the PTEN measuring; and administering a therapeutic agent for treating prostate cancer to the subject from which the prostate cancer sample was obtained, performing a prostatectomy on the subject from which the prostate cancer sample was obtained, or combinations thereof. ### Claim 2 (depends on 1) The method of claim 1, wherein the prostate cancer sample is a prostate cancer sample that has not penetrated the prostatic capsule, and the method determines that the risk that the prostate cancer will penetrate the prostatic capsule in the future is at least four-times more likely when increased expression of ERG and decreased expression of PTEN is measured in the prostate cancer sample relative to the control. ### Claim 3 (depends on 1) The method of claim 1, wherein it is not known whether the prostate cancer sample has penetrated the prostatic capsule prior to performing the method, and the method determines that the cancer has penetrated the prostatic capsule when increased expression of ERG and decreased expression of PTEN is measured in the prostate cancer sample relative to the control. ### Claim 4 (depends on 1) The method of claim 1, further comprising: measuring expression of one or more other prostate cancer-related molecules in the sample; and comparing expression of the one or more other prostate cancer related molecules in the prostate cancer sample to a control representing expression of the one or more other prostate cancer-related molecules expected in a normal prostate sample. ### Claim 5 (depends on 1) The method of claim 1, wherein the prostate cancer sample is a fixed, wax-embedded prostate cancer tissue sample. ### Claim 6 (depends on 1) The method of claim 1, wherein the prostate cancer sample is collected after prostate cancer diagnosis and after prostatectomy in the subject. ### Claim 7 (depends on 1) The method of claim 1, wherein the prostate cancer sample is collected from tissue removed during a prostatectomy. ### Claim 8 (depends on 1) The method of claim 1, wherein the ERG-specific antibody is a rabbit monoclonal antibody produced from hybridoma clone EPR 3864 and the PTEN-specific antibody is a rabbit monoclonal antibody produced from hybridoma clone 138G6. ### Claim 9 (depends on 1) The method of claim 1, further comprising generating a report, wherein the report comprises a risk that a prostate cancer will penetrate the prostatic capsule. ### Claim 10 (depends on 1) The method of claim 1, wherein the therapeutic agent is radiation, a chemotherapeutic, or a hormone. ### Claim 11 (depends on 10) The method of claim 10, wherein the chemotherapeutic comprises temozolomide or docetaxel.