Back to results
US 9,435,810 B2Granted
Molecules and methods for iterative polypeptide analysis and processing
AssigneeWashington University
InventorsJames J. Havranek, Benjamin Borgo
Life of this patent
Estimating the term…
Abstract
Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.
Claims20
A method for identifying a N-terminal amino acid of a polypeptide, the method comprising:
- (a) contacting the polypeptide with one or more fluorescently labeled N-terminal amino acid binding proteins (NAABs);
- (b) detecting fluorescence of the NAAB that bound to the N-terminal amino acid of the polypeptide;
- and
- (c) identifying the N-terminal amino acid of the polypeptide based on the fluorescence detected.
1 more element
A method for cleaving the N-terminal amino group of a polypeptide, the method comprising:
- (a) reacting the N-terminal amino acid of the polypeptide with phenyl isothiocyanate (PITC) to generate a PITC-derivatized N-terminal amino acid;
- and
- (b) cleaving the modified N-terminal amino group with an Edman degradation enzyme.
Dependencies are inferred from the claim text.