# US 9,435,800 B2 · Sample assembly with an electromagnetic field to accelerate the bonding of target antigens and nanoparticles

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- Number: US 9,435,800 B2
- Document ID: US9435800B2
- Status: Granted
- Kind: B2
- Application number: 13/616,855
- Filed: 2012-09-14
- Published: 2014-03-20
- Granted: 2016-09-06
- Earliest claimed: 2012-09-14
- Assignees: International Business Machines Corporation
- Applicants: Allen K. Bates; Nils Haustein; Stephen L. Schwartz; Anna W. Topol; Daniel J. Winarski
- Inventors: Allen K. Bates; Nils Haustein; Stephen L. Schwartz; Anna W. Topol; Daniel J. Winarski
- Web page: https://patentgrep.com/patent/US9435800B2

## CPC

- G01N 33/54386: {Analytical elements}
- G01N 33/54326: {Magnetic particles}
- G01N 33/54333: {Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction}
- G01N 33/54346: {Nanoparticles}
- G01N 33/54353: {with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393)}
- G01N 33/54366: {Apparatus specially adapted for solid-phase testing}
- G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding}
- B82Y 5/00: Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
- B82Y 15/00: Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
- G01N 15/1031: by measuring electrical or magnetic effects
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

Described are embodiments of an invention for a sample assembly with an electrical conductor for generating an electromagnetic field to speed up the tagging of target antigens with antiparticles for detection of the antigens by electromagnetic read heads. A sample assembly includes a surface with a first set of antibodies bonded thereon. Target antigens are bonded with the first set of antibodies. A second set of antibodies bonded to nanoparticles are exposed to the sample surface to bond with the target antigens. The electrical conductor generates an electromagnetic field that moves the nanoparticle-labeled antibodies toward the antigens to shorten the time to complete their bonding process.

## Claims

### Claim 1

A sample assembly comprising:
a substrate;
one or more base layers formed on the substrate;
an outer layer formed on the substrate;
a plurality of sample trenches formed in the outer layer, each sample trench being characterized by an upper surface, a bottom surface, and a longitudinal axis;
a first set of antibodies bonded to the sample assembly via the bottom surface of one or more of the sample trenches; and
an electrical conductor disposed in the substrate, the electrical conductor being configured to generate an electromagnetic field in proximity to the plurality of sample trenches to enhance nanoparticle movement toward the bottom surface of the plurality of sample trenches; and
at least one alignment trench formed in the outer layer, the alignment trench having a longitudinal axis substantially parallel to the longitudinal axis of at least one of the sample trenches, each alignment trench comprising non-magnetic markings configured to facilitate alignment of an electromagnetic write head of a magnetic tape drive.

### Claim 2 (depends on 1)

The sample assembly of claim 1, further comprising: a biological sample in one or more of the sample trenches, the biological sample comprising:
one or more target antigens; and
a second set of antibodies, wherein the second set of antibodies are bound to the nanoparticles,
wherein the second set of antibodies are characterized by binding with the target antigens, and
wherein the first and second sets of antibodies are biologically identical.

### Claim 3 (depends on 1)

The sample assembly of claim 1, wherein the electrical conductor is entirely disposed below an upper surface of the substrate, and wherein the electrical conductor comprises a material selected from gold, copper, aluminum and silver.

### Claim 4 (depends on 1)

The sample assembly of claim 1, further comprising an external magnet not physically coupled to the sample assembly,
wherein the external magnet is located in proximity to the sample assembly, wherein the external magnet is positioned to increase a magnitude of the electromagnetic field and further facilitate the nanoparticle movement toward the bottom surface of the plurality of sample trenches.

### Claim 5 (depends on 1)

The sample assembly of claim 1, wherein the at least one alignment trench comprises a plurality of the alignment trenches, wherein each alignment trench is formed in the outer layer.

### Claim 6 (depends on 2)

The sample assembly of claim 2, wherein the nanoparticles are bound to the target antigens.

### Claim 7 (depends on 1)

The sample assembly of claim 1, wherein the nanoparticles are magnetized.

### Claim 8 (depends on 1)

The sample assembly of claim 1, wherein the plurality of sample trenches and the at least one alignment trench are each characterized by a substantially linear shape trenches.

### Claim 9

A method of forming a sample assembly of a biological sample having target antigens, the method comprising:
providing a substrate having a surface and an embedded electrical conductor;
bonding a first set of antibodies on the substrate surface;
exposing the substrate surface with the first set of bonded antibodies to the biological sample having the target antigens, wherein the target antigens bond with the first set of antibodies on a bottom surface of a plurality of sample trenches, each sample trench having a substantially linear shape and being formed in an outer layer disposed on the substrate;
forming one or more alignment trenches in the outer layer;
bonding a second set of antibodies to nanoparticles, wherein the first and second sets of antibodies are biologically identical;
exposing the target antigens bonded with the first set of antibodies to the second set of antibodies bonded with the nanoparticles, wherein the second set of antibodies bond with the target antigens; and
applying an electric current through the electrical conductor to generate an electromagnetic field, wherein the electromagnetic field generated by applying the electric current through the electrical conductor comprises an electrical component and a magnetic component,
wherein each alignment trench comprises non-magnetic markings configured to facilitate alignment of an electromagnetic write head of a magnetic tape drive,
wherein the magnetic component of the electromagnetic field moves the nanoparticles toward the bottom surface of the plurality of sample trenches, and
wherein moving the nanoparticles toward the bottom surface of the plurality of sample trenches speeds up the bonding between the second set of antibodies and the target antigens.

### Claim 10

A method of detecting target antigens in a biological sample on a sample assembly, the sample assembly including a substrate having a surface and an electrical conductor, the method comprising:
bonding a first set of antibodies on the substrate surface exposed via one or more sample trenches formed in an outer layer formed on the substrate surface, each sample trench having a longitudinal axis and a substantially linear shape arranged along the longitudinal axis thereof;
exposing the substrate surface with the first set of bonded antibodies to the biological sample having the target antigens, wherein the target antigens bond with the first set of antibodies;
bonding a second set of antibodies to nanoparticles, wherein the first and second sets of antibodies are biologically identical;
exposing the target antigens bonded with the first set of antibodies to the second set of antibodies bonded with the nanoparticles, wherein the second set of antibodies bond with the target antigens;
applying an electric current through the electrical conductor to generate an electromagnetic field, wherein the electromagnetic field generated by applying the electric current through the electrical conductor comprises an electrical component and a magnetic component, wherein the magnetic component of the electromagnetic field moves the nanoparticles toward the substrate surface, and wherein moving the nanoparticles toward the substrate surface speeds up the bonding between the second set of antibodies and the target antigens;
magnetizing the nanoparticles using an electromagnetic write head;
reading the magnetized nanoparticles using a magneto-resistive read sensor to detect the target antigens; and
aligning at least one of the electromagnetic write head and the electromagnetic read sensor with at least one of the sample trenches using at least one alignment trench also formed in the outer layer, each alignment trench having a longitudinal axis and a substantially linear shape arranged along the longitudinal axis thereof.

### Claim 11 (depends on 10)

The method of claim 10, wherein the electromagnetic write head and the magneto-resistive read sensor are parts of a read-write head module in a data storage system.

### Claim 12 (depends on 10)

The method of claim 10, further comprising bonding the first set of antibodies to the substrate surface and bonding the second set of antibodies to the nanoparticles using a material selected from the group consisting of amide, self-assembled-monolayers (SAMS), alkoxysilane, organic functional trialkoxysilane, and thiol.

### Claim 13 (depends on 10)

The method of claim 10, wherein the at least one alignment trench and the one or more sample trenches are substantially parallel along the longitudinal axes thereof.

### Claim 14 (depends on 10)

The method of claim 10, wherein applying the electromagnetic field through the electrical conductor to generate the electromagnetic field moves the nanoparticles toward the sample surface at a rate about two orders of magnitude greater than a rate at which the nanoparticles move toward the sample surface in absence of applying the electrical current through the electrical conductor to generate the electromagnetic field.

### Claim 15 (depends on 1)

The sample assembly of claim 1, the outer layer comprising one or more of:
silicon dioxide; and
a low-friction material selected from: aluminum oxide (Al2O3), diamond-like carbon (DLC) and polyamide.

### Claim 16 (depends on 1)

The sample assembly of claim 1, each sample trench being substantially linear along a longitudinal axis thereof, wherein the plurality of sample trenches are substantially parallel along the longitudinal axes thereof.

### Claim 17 (depends on 1)

The sample assembly of claim 1, wherein the one or more base layers are arranged in a single layer formed on the substrate.

### Claim 18 (depends on 1)

The sample assembly of claim 1, further comprising a plurality of the alignment trenches, each alignment trench:
having a longitudinal axis and a substantially linear shape arranged along the longintudinal axis thereof;
formed adjacent to at least one and at most two of the sample trenches; and
parallel to the longitudinal axes of the plurality of sample trenches.

### Claim 19 (depends on 10)

The method of claim 10, wherein the at least one alignment trench comprises non-magnetic markings configured to facilitate alignment of an electromagnetic write head of a magnetic tape drive.