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US 9,435,787 B2Granted
Cell analysis by mass cytometry
AssigneeFluidigm Canada Inc.
InventorVladimir Baranov
Life of this patent
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Abstract
A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.
Claims13
A method for cellular analysis by mass cytometry comprising:
- providing a group of cells labeled with more than one distinct elemental tag;
- selecting a candidate cell in the group of cells by identifying a location at the candidate cell, the candidate cell having a property of interest;
- recording the location at the candidate cell;
- directing at least one laser pulse onto the candidate cell at the recorded location and generating a discrete plume for each of the at least one laser pulse, each of the discrete plume comprises the more than one distinct elemental tag;
- introducing each of the discrete plume into an inductively coupled plasma and generating groups of elemental ions corresponding with each of the more than one distinct elemental tag;
- detecting each of the groups of elemental ions simultaneously for each of the discrete plume by mass cytometry;
- and
- correlating the detected elemental ions with the property of interest.
5 more elements
Dependencies are inferred from the claim text.