# US 9,435,720 B2 · Multiple slide processing apparatus

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- Number: US 9,435,720 B2
- Document ID: US9435720B2
- Status: Granted
- Kind: B2
- Application number: 14/807,990
- Filed: 2015-07-24
- Published: 2016-07-21
- Granted: 2016-09-06
- Earliest claimed: 2013-04-05
- Assignees: Hardy Diagnostics
- Applicants: Hardy Diagnostics
- Inventors: Jerry West; Randy Thomas; Jeff Gibbs; Lee Carter
- Web page: https://patentgrep.com/patent/US9435720B2

## CPC

- G01N 1/30: Staining; Impregnating {; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis}
- G01N 1/312: {for samples mounted on planar substrates}
- G01N 35/00029: {provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence)}
- G01N 35/0099: {comprising robots or similar manipulators (robots per se B25J)}
- G01N 2001/302: {Stain compositions}
- G01N 2035/00039: {Transport arrangements specific to flat sample substrates, e.g. pusher blade}
- G01N 2035/00138: {Slides}
- G01N 2035/0094: {optimisation; experiment design}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A slide processing apparatus comprises a slide or processing station which may include a plurality of cuvettes, each cuvette configured to receive a slide. A reagent fluid supply is coupled to each cuvette to deliver and apply reagent for treatment of the slide. A slide gripper is disposed above the slide processing station and is configured to move the slide in and out of the cuvettes. A screw assembly is disposed adjacent the slide processing station, the screw assembly configured to receive a plurality of slides, wherein rotation of the screw assembly advances the plurality of slides for engagement by the slide gripper.

## Claims

### Claim 1

A method of sequentially treating microscope slides using treatment processes of different duration comprising the steps of:
placing a plurality of microscope slides in a corresponding plurality of first-process receptacles for treatment with a first treatment process;
removing a first of the plurality of microscope slides from one of the first-process receptacles, creating a vacated receptacle;
placing the first microscope slide in a second-process receptacle for treatment with a second treatment process, while other of the plurality of microscope slides undergo treatment with the first treatment process, wherein the second treatment process is of shorter duration than the first treatment process;
replacing the first microscope slide with a new microscope slide in the vacated first-process receptacle;
removing the first microscope slide from the second-process receptacle; and
replacing the first microscope slide in the second-process receptacle with another of the plurality of microscope slides from the first-process receptacles.

### Claim 2 (depends on 1)

The method according to claim 1, further comprising the steps of:
after removing the first of the plurality of microscope slides from the second-process receptacle, placing the first microscope slide in a third-process receptacle for treatment with a third treatment process.

### Claim 3 (depends on 1)

The method according to claim 1, wherein:
the first treatment process is application of Gram's iodine to the microscope slide, and occurs in two first-process receptacles;
the second treatment process is application of acid alcohol to the microscope slide and occurs in one second-process receptacle, and the application of acid alcohol is approximately half the duration of the application of Gram's iodine; and
the third treatment process is application of safranin to the microscope slide and occurs in two third-process receptacles.

### Claim 4 (depends on 3)

The method according to claim 3, further comprising:
prior to the step of placing the plurality of microscope slides into the corresponding plurality of first-process receptacles, placing the plurality of slides into a pair of crystal violet receptacles for the application of crystal violet.

### Claim 5

A method of sequentially Gram staining microscope slides using treatment processes of different duration comprising the steps of:
placing a plurality of microscope slides in a corresponding plurality of Gram's iodine receptacles for treatment with Gram's iodine;
removing a first of the plurality of microscope slides from one of the Gram's iodine receptacles, creating a vacated receptacle;
placing the first microscope slide in an acid-alcohol receptacle for treatment with acid alcohol, while other of the plurality of microscope slides undergo treatment with the Gram's iodine, wherein the acid alcohol treatment is of shorter duration than the Gram's iodine treatment;
replacing the first microscope slide with a new microscope slide in the vacated Gram's iodine receptacle;
removing the first microscope slide from the acid alcohol receptacle; and
replacing the first microscope slide in the acid alcohol receptacle with another of the plurality of microscope slides from the Gram's iodine receptacles.

### Claim 6 (depends on 5)

The method according to claim 5, further comprising the steps of:
after removing the first of the plurality of microscope slides from the Gram's iodine receptacle, placing the first microscope slide in a third-process receptacle for treatment with a third treatment process.

### Claim 7 (depends on 6)

The method according to claim 6, wherein:
the third treatment process is application of safranin to the microscope slide and occurs in two third-process receptacles.

### Claim 8 (depends on 5)

The method according to claim 5, further comprising the step of:
prior to the step of placing the plurality of microscope slides into the corresponding plurality of Gram's iodine receptacles, placing the plurality of slides into a pair of crystal violet receptacles for application of crystal violet.