# US 9,434,993 B2 · Systems for identifying cosmetic agents for skin care compositions

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- Number: US 9,434,993 B2
- Document ID: US9434993B2
- Status: Granted
- Kind: B2
- Application number: 14/749,717
- Filed: 2015-06-25
- Published: 2015-10-15
- Granted: 2016-09-06
- Earliest claimed: 2011-02-22
- Assignees: The Procter & Gamble Company
- Applicants: The Procter & Gamble Company
- Inventors: Robert Lloyd Binder; Robert Scott Youngquist; Jun Xu; Kenton Duane Juhlin; Rosemarie Osborne; Scott Michael Hartman
- Web page: https://patentgrep.com/patent/US9434993B2

## CPC

- C12Q 1/6883: for diseases caused by alterations of genetic material
- G16B 20/00: ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B 20/20: Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
- G16B 20/30: Detection of binding sites or motifs
- G16B 25/00: ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B 25/10: Gene or protein expression profiling; Expression-ratio estimation or normalisation
- G16B 50/00: ICT programming tools or database systems specially adapted for bioinformatics
- G16B 50/30: Data warehousing; Computing architectures
- C12Q 2600/148: Screening for cosmetic compounds
- C12Q 2600/158: Expression markers
- G16B 25/20: Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G16B: BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY

## Abstract

A system for identifying connections between a cosmetic agent and at least one gene associated with a skin aging condition. The system includes a computer readable medium having stored thereon a plurality of instances and a skin aging gene expression signature. Each instance comprises an ordered list of identifiers representing a plurality of differentially expressed genes generated as a result of contact between the cosmetic agent and a human fibroblast cell or a human keratinocyte cell. The computing device includes computer-readable instructions for accessing the plurality of instances and the skin aging gene expression signature stored on the computer readable medium, comparing each of the instances to the skin aging gene expression signature, and assigning a connectivity score to each of the instances based on the comparison.

## Claims

### Claim 1

A system for identifying connections between a cosmetic agent and at least one gene associated with a skin aging condition, comprising:
a. at least one computer readable medium having stored thereon a plurality of instances and a skin aging gene expression signature, wherein each instance comprises an ordered list of identifiers representing a plurality of up-regulated and a plurality of down regulated genes differentially expressed in response to contact between the cosmetic agent and a human fibroblast cell or a human keratinocyte cell, the skin aging gene expression signature comprises one or more lists comprising a plurality of identifiers representing a plurality of up-regulated genes and a plurality of down-regulated genes associated with a skin aging condition, and from about 80% to about 100% of the plurality of identifiers associated with the skin aging gene expression signature represent a gene set forth in any of Tables A to D; and
b. a first computing device comprising computer-readable instructions that cause the computing device to:
i. access the plurality of instances and the skin aging gene expression signature stored on the computer readable medium;
ii. comparing each of the instances to the skin aging gene expression signature, wherein the comparison comprises comparing each identifier in the gene expression signature list(s) with the position of the same identifier in the instance list; and
iii. assigning a connectivity score to each of the plurality of instances based on the comparison in (ii).

### Claim 2 (depends on 1)

The system of claim 1, further comprising a microarray having a plurality of probes for hybridizing to cDNA reverse transcribed from mRNA extracted from the fibroblast cell or the keratinocyte cell.

### Claim 3 (depends on 2)

The system of claim 2, further comprising a microarray scanner for scanning the microarray and translating the plurality of hybridized probes to gene expression data.

### Claim 4 (depends on 3)

The system of claim 3, further comprising a second computing device for transmitting the gene expression data from the microarray scanner to the first computing device.

### Claim 5 (depends on 2)

The system of claim 2, further comprising a plurality of cosmetic agents for application to the fibroblast cell or the keratinocyte cell.

### Claim 6 (depends on 5)

The system of claim 5, wherein the plurality of cosmetic agents comprise a botanical derived from a root, stem, bark, leaf, seed or fruit of a plant.

### Claim 7 (depends on 1)

The system of claim 1, wherein the plurality of identifiers are selected from the group consisting of a microarray probe set ID, a human gene name, a human gene symbol and combinations thereof.

### Claim 8 (depends on 7)

The system of claim 7, wherein the plurality of identifiers comprises from about 50 to about 600 identifiers.

### Claim 9 (depends on 8)

The system of claim 8, wherein the plurality of identifiers associated with the at least one gene expression signature are selected from the genes of Tables C and D.

### Claim 10 (depends on 8)

The system of claim 8, wherein the plurality of identifiers associated with the at least one gene expression signature are selected from the genes of Tables A and B.

### Claim 11 (depends on 1)

The system of claim 1, wherein each of the plurality of instances further comprises metadata associated with the fibroblast cell or the keratinocyte cell and the cosmetic agent.

### Claim 12 (depends on 1)

The system of claim 1, wherein the plurality of instances comprises between about 50 and about 50,000 instances.

### Claim 13 (depends on 12)

The system of claim 12, wherein the plurality of instances comprises between about 1,000 and about 20,000 instances.

### Claim 14 (depends on 1)

The system of claim 1, further comprising a full thickness skin biopsy from skin having a skin aging condition associated with the at least one skin aging gene expression signature.

### Claim 15 (depends on 14)

The system of claim 14, wherein the full thickness skin biopsy is from skin that is sun protected.

### Claim 16 (depends on 15)

The system of claim 15, wherein where the full thickness skin biopsy is from an individual having an age from about 45 years to about 80 years.

### Claim 17 (depends on 15)

The system of claim 15, further comprising a second full thickness skin biopsy from an individual having an age from about 18 years to about 25 years.