# US 9,434,969 B2 · Manufacture of amides

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- Number: US 9,434,969 B2
- Document ID: US9434969B2
- Status: Granted
- Kind: B2
- Application number: 12/879,558
- Filed: 2010-09-10
- Published: 2011-01-13
- Granted: 2016-09-06
- Earliest claimed: 2003-12-02
- Assignees: BASF SE
- Applicants: Yvonne Armitage; Jatinder Singh Kullar
- Inventors: Yvonne Armitage; Jatinder Singh Kullar
- Web page: https://patentgrep.com/patent/US9434969B2

## CPC

- C12P 13/02: Amides, e.g. chloramphenicol {or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes (peptides C12P21/00 or C07K)}
- Y10S 435/822: using bacteria or actinomycetales
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

A method of producing an amide from the corresponding nitrile comprising the following steps, i) providing a microorganism capable of producing a nitrile hydratase biocatalyst, ii) culturing the microorganism in a growth medium, iii) storing the microorganism, iv) contacting the nitrile by the microorganism in an aqueous medium and thereby converting the nitrile to the amide, wherein the microorganism is stored as none actively growing free cells in a storage medium that comprises water. The stored microorganism may be as whole microbial cells, this may be in the form of a cell paste recovered from a fermentation medium; an aqueous suspension of the microbial cells, prepared using a suitable suspending medium such as water, physiological saline solution, or a suitable buffer solution, such as phosphate or comprises the fermentation broth that contains components of the fermentation culture medium and products of microbial culture. The microorganism exhibits no significant loss of activity, for example if stored even for at least 2 days, especially 3 to 28 days.

## Claims

### Claim 1

A method of producing an amide from the corresponding nitrile comprising the following steps,
i) providing a microorganism of the genus Rhodococcus capable of producing a nitrile hydratase biocatalyst,
ii) culturing the microorganism in a growth medium,
iii) storing the microorganism,
iv) contacting the nitrile with the microorganism in an aqueous medium and thereby converting the nitrile to the amide,
wherein the microorganism is retained in the growth medium and the microorganism is not recovered from the original fermentation medium and stored without further downstream processing steps at a temperature above the freezing point to 25° C. as non-actively growing free cells in the growth medium that comprises water and urea or a urea derivative.

### Claim 2 (depends on 1)

A method according to claim 1 in which the amide is an ethylenically unsaturated amide.

### Claim 3 (depends on 1)

A method according to claim 1 in which the microorganism is stored for a period of at least 2 days.

### Claim 4 (depends on 1)

A method according to claim 1 in which the microorganism is Rhodococcus rhodochrous NCIMB 41164.

### Claim 5 (depends on 1)

A method according to claim 1 in which the microorganism is of the Rhodococcus rhodochrous species.

### Claim 6 (depends on 3)

A method according to claim 3, wherein the microorganism is stored for a period between 3 and 28 days.

### Claim 7 (depends on 1)

A method according to claim 1, wherein the microorganism is stored between 5 and 25° C.