# US 9,434,967 B2 · Microbial culture medium and cultural method

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 9,434,967 B2
- Document ID: US9434967B2
- Status: Granted
- Kind: B2
- Application number: 14/041,473
- Filed: 2013-09-30
- Published: 2014-04-03
- Granted: 2016-09-06
- Earliest claimed: 2012-09-28
- Assignees: Enn Science & Technology Development Co., Ltd.
- Applicants: ENN Science and Technology Development Co., Ltd.
- Inventors: Minsheng Liu; Qian Feng; Zhongzhen Cai; Lin Wang
- Web page: https://patentgrep.com/patent/US9434967B2

## CPC

- C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention discloses a microbial culture medium and a cultural method. The culture medium comprises a combination of strong acid-weak alkali salts and strong alkali-weak acid salts so that pH value thereof can be regulated and controlled in a stable and suitable range by itself, thus decreasing or eliminating an operable regulation on pH value via an external device in fermentation process and a risk of contaminations resulted from it in fermentation process as well, accordingly greatly improving the controllability and stability of the fermentation process. Meanwhile the ammonium salts acting as strong acid-weak alkali salts can be used as a nitrogen source to replace yeast cream to realize a decreased amount of nitrogen resource of high price, therefor sharply reducing the cost of the culture medium.

## Claims

### Claim 1

A fermentation medium for algae comprising
a combination of strong acid-weak alkali salts and strong alkali-weak acid salts, wherein said strong acid-weak alkali salts are selected from ammonium nitrate, ammonium sulfate, ammonium chloride and ammonium oxalate or a mixture of at least two thereof in an amount of 3 to 5 g/L; and said strong alkali-weak acid salts are sodium, potassium or calcium salts of glutamic acid, aspartic acid, lysine, arginine, histidine, glycine, serine, threonine, cysteine, tyrosine, alanine, valine, leucine, isoleucine, auxiliary leucine, phenylalanine, tryptophan, methionine or a mixture of at least two thereof in an amount of 5 to 20 g/L, and further comprising 10 to 200 g/L of glucose, 5 to 20 g/L of yeast cream, 0.1 to 35 g/L of inorganic salts, 10 to 30 ppm of vitamin B1, 5 to 15 ppm of vitamin B6, 1 to 5 ppm of vitamin B 12, 1 to 5 ppm of vitamin H, and 3 to 15 ppm of 6-benzyl amino adenine.

### Claim 2 (depends on 1)

The medium of claim 1, characterized in that said combination of strong acid-weak alkali salts and strong alkali-weak acid salts controls the pH value of the medium in range from 4.0 to 9.0.

### Claim 3 (depends on 1)

The medium of claim 1, characterized in that said strong alkali-weak acid salt is sodium glutamate.

### Claim 4 (depends on 1)

The medium of claim 1, further comprising: 5 to 20 g/L of sodium glutamate, and 3.5 to 5 g/L of ammonium sulfate.

### Claim 5 (depends on 1)

The culture medium of claim 1, characterized in that said combination of strong acid-weak alkali salts and strong alkali-weak acid salts controls the pH value of the medium in range from 4.5 to 6.0.