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US 9,434,966 B2Granted
Biological methods for preparing adipic acid
AssigneeVerdezyne, Inc.
InventorsStephen Picataggio, Tom Beardslee
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Abstract
The technology relates in part to biological methods for producing adipic acid and engineered microorganisms capable of such production.
Claims18
A method for producing adipic acid, comprising:
- (a) contacting a Candida yeast with a feedstock comprising a vegetable oil, wherein the Candida yeast comprises:
- (i) a genetic alteration that partially blocks beta oxidation activity of acyl-CoA oxidase polypeptide,
- (ii) a genetic alteration that reduces or eliminates long chain acyl-CoA synthetase activity, and
- (iii) a genetic alteration that reduces or eliminates acyl-CoA synthetase activity,
- wherein the genetic alteration of (i) reduces the amount of a polypeptide having an acyl-CoA oxidase activity, and the polypeptide is a POX4 polypeptide or a POX5 polypeptide;
- (b) culturing the Candida yeast under conditions in which adipic acid is produced.
3 more elements
Dependencies are inferred from the claim text.