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US 9,434,960 B2Granted
Methods for making and using modified oocytes
AssigneeNew York Stem Cell Foundation, Inc.
InventorDietrich M. Egli
Life of this patent
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Abstract
The present invention provides modified oocytes having a nuclear genome derived from a first oocyte and cytoplasm derived from a second oocyte from a different subject, and methods for making and using such modified oocytes. The methods and compositions of the present invention can be useful in a variety of settings including, but not limited to, in in vitro fertilization (“IVF”) procedures.
Claims33
A method for producing a modified mammalian oocyte, comprising:
- introducing a nuclear genome from a first mammalian oocyte into an enucleated second mammalian oocyte to form a modified mammalian oocyte, wherein the nuclear genome is from a metaphase I mammalian oocyte or an immature metaphase II mammalian oocyte, and wherein the first oocyte and the second oocyte are from mammals of the same species, thereby producing a modified mammalian oocyte capable of developing at least to blastocyst stage.
A method for producing a modified mammalian oocyte comprising:
- a) treating a karyoplast comprising a nuclear genome from a first mammalian oocyte to induce partial spindle depolymerization, wherein the nuclear genome is a mature metaphase II nuclear genome, and
- b) introducing into an enucleated second mammalian oocyte the karyoplast comprising the partially depolymerized spindle, thereby producing a modified mammalian oocyte capable of developing at least to blastocyst stage,
- wherein the first oocyte and the second oocyte are from mammals of the same species.
Dependencies are inferred from the claim text.