# US 9,434,955 B2 · Proteins relating to grain shape and leaf shape of rice, coding genes and uses thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,434,955 B2 - Document ID: US9434955B2 - Status: Granted - Kind: B2 - Application number: 13/391,993 - Filed: 2010-07-08 - Published: 2012-09-20 - Granted: 2016-09-06 - Earliest claimed: 2009-08-24 - Assignees: Institute of Subtropical Agriculture, Chinese Acad - Applicants: Xinjie Xia - Inventors: Xinjie Xia - Web page: https://patentgrep.com/patent/US9434955B2 ## CPC - C12N 15/8261: {with agronomic (input) traits, e.g. crop yield} - C07K 14/415: from plants - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract The present invention provides a protein OsXCL relating to grain shape and leaf shape of rice, its derived proteins and their coding genes. Transgenic rices over-expressing OsXCL gene present phenotypes as increase of grain length, grain weight and number of grains per panicle, and leaf rolling and so on. The present invention also provides the method for obtaining transgenic plants by transforming the coding genes of OsXCL or its derived proteins into objective plants. ## Claims ### Claim 1 A method for increasing one or more of grain weight, grain length, grain number per panicle and/or rolled leaves of a plant, which comprises: introducing a recombinant expression vector comprising a nucleic acid encoding the protein as set forth by SEQ ID NO. 2 into a target plant; screening the transgenic plant for the recombinant expression vector; and breeding the transgenic plant wherein the plant exhibits increased grain weight, increased grain length, increased grain number per panicle and/or rolled leaves compared to a non-transgenic plant. ### Claim 2 (depends on 1) The method according to claim 1, wherein the plant is a dicotyledonous plant or a monocotyledonous plant. ### Claim 3 A method for increasing one or more of grain weight, grain length, grain number per panicle and/or rolled leaves of a plant, comprising: regenerating a transgenic cell line or breeding a recombinant plant with another plant in a selection medium to obtain a transgenic plant, wherein the cell line or recombinant plant comprises a heterologous nucleic acid selected from a) a nucleic acid encoding the polypeptide that is encoded by nucleotides 106-870 of SEQ ID NO. 1; b) a nucleic acid encoding the polypeptide that is encoded by nucleotides 50-873 of SEQ ID NO. 1; and selecting and growing a plant with increased grain weight, increased grain length, increased grain number per panicle and/or rolled leaves of the transgenic plant with compared to a non-transgenic plant. ### Claim 4 A method for increasing one or more of grain weight, grain length, grain number per panicle and/or rolled leaves of a plant, which comprises: introducing a recombinant expression vector comprising a nucleic acid selected from: a) a nucleic acid encoding the polypeptide that is encoded by positions 106-870 from the 5′ end of SEQ ID NO. 1; b) a nucleic acid encoding the polypeptide that is encoded by positions 50-873 from the 5′ end of SEQ ID NO. 1; into a target plant; screening the transgenic plant for the recombinant expression vector; and identifying and breeding the transgenic plant wherein the plant exhibits increased grain weight, increased grain length, increased grain number per panicle and/or rolled leaves compared to a non-transgenic plant. ### Claim 5 (depends on 4) The method according to claim 4, wherein the plant is a dicotyledonous plant or a monocotyledonous plant. ### Claim 6 (depends on 4) The method according to claim 4, wherein the recombinant expression vector is obtained by: inserting the nucleic acid into a multiple cloning site of the expression vector 163-1300; wherein, the construction method of the expression vector 163-1300 is: ligating a DNA band containing Double 35S promoter produced by cleaving enzymatically pJIT 163 with Kpnl and Xhol with a large fragment produced by cleaving enzymatically pCAMBIA1300 with Kpnl and Sail to give the recombinant expression vector; transforming the recombinant expression vector into a host cell; and identifying and recovering the recombinant vector with the inserted nucleic acid. ### Claim 7 (depends on 1) The method according to claim 1, wherein the recombinant expression vector is obtained by: inserting the nucleic acid into a multiple cloning site of the expression vector 163-1300; wherein, the construction method of the expression vector 163-1300 is: ligating a DNA band containing Double 35S promoter produced by cleaving enzymatically pJIT 163 with Kpnl and Xhol with a large fragment produced by cleaving enzymatically pCAMBIA1300 with Kpnl and Sall to give the recombinant expression vector, transforming the recombinant expression vector into a host cell; and identifying and recovering the recombinant vector with the inserted nucleic acid. ### Claim 8 (depends on 2) The method according to claim 2, wherein the a dicotyledonous plant is Arabidopsis thaliana, and the a monocotyledonous plant is rice. ### Claim 9 (depends on 5) The method according to claim 5, wherein the a dicotyledonous plant is Arabidopsis thaliana, and the a monocotyledonous plant is rice.