# US 9,434,942 B2 · Small interfering RNAS with target-specific seed sequences

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- Number: US 9,434,942 B2
- Document ID: US9434942B2
- Status: Granted
- Kind: B2
- Application number: 13/980,714
- Filed: 2012-02-14
- Published: 2013-12-12
- Granted: 2016-09-06
- Earliest claimed: 2011-02-14
- Assignees: Syngenta Participations AG
- Applicants: Xiang Huang; Thomas McNeill; Mike Schweiner
- Inventors: Xiang Huang; Thomas McNeill; Mike Schweiner
- Web page: https://patentgrep.com/patent/US9434942B2

## CPC

- C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
- C12Q 1/6888: for detection or identification of organisms
- C12N 15/113: Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)}
- C12N 15/8282: {for fungal resistance}
- C12N 15/8285: {for nematode resistance}
- C12Q 1/6895: for plants, fungi or algae
- C12N 2310/113: targeting other non-coding nucleic acids, e.g. antagomirs
- C12N 2330/31: Libraries, arrays
- C12Q 2600/13: Plant traits
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Disclosed are methods for design and synthesis of siRNA libraries, siRNA libraries produced thereby, siRNA molecules, and uses thereof.

## Claims

### Claim 1

A siRNA molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 1-14 and wherein the siRNA molecule reduces the number of soybean cyst nematode cysts on soybean roots.

### Claim 2 (depends on 1)

An artificial RNA molecule comprising the siRNA molecule of claim 1, wherein the artificial RNA molecule is encoded by the nucleotide sequence of any one of SEQ ID NOs: 16-29.

### Claim 3 (depends on 1)

A vector comprising the siRNA molecule of claim 1, wherein the vector is encoded by the nucleotide sequence of any one of SEQ ID NOs: 31-44.