# US 9,434,929 B2 · Esterases useful in the treatment of cellulosic and lignocellulosic material

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- Number: US 9,434,929 B2
- Document ID: US9434929B2
- Status: Granted
- Kind: B2
- Application number: 14/060,999
- Filed: 2013-10-23
- Published: 2014-05-01
- Granted: 2016-09-06
- Earliest claimed: 2012-10-26
- Assignees: Roal Oy
- Applicants: Roal Oy
- Inventors: Kristiina Jarvinen; Kari Juntunen; Taija Leinonen; Alexandra Komander; Kim Langfelder; Jari Vehmaanpera; Terhi Puranen
- Web page: https://patentgrep.com/patent/US9434929B2

## CPC

- C12N 9/18: Carboxylic ester hydrolases {(3.1.1)}
- A21D 8/042: {with enzymes}
- A23K 20/189: Enzymes
- A23L 29/06: {Enzymes}
- C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase}
- C12N 1/205: Bacterial isolates
- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- C12Y 301/01073: Feruloyl esterase (3.1.1.73)
- C12R 2001/19: Escherichia coli
- Y02P 20/52: using catalysts, e.g. selective catalysts
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A21D: TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS (preservation of flour or dough before baking A23B40/00; preservation of bakery products A23B45/00)
- A23K: FODDER
- A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12Y: ENZYMES
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- Y02P: CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

## Abstract

The present invention discloses novel polypeptides and enzyme preparations containing them, which improve the efficiency of cellulose and lignocellulose degradation even at elevated temperatures. The polypeptides can be produced using conventional recombinant DNA technologies. The related polynucleotides, vectors and host cells are also disclosed. The polypeptides and the enzyme preparations containing them are particularly useful in improving the efficiency of cellulose and lignocellulose degradation, in improving the quality of animal feed, in machine dishwashing applications, in detergent compositions, in pulp and paper, textile, food, baking or beverage industry.

## Claims

### Claim 1

A detergent composition comprising an enzyme preparation comprising a recombinant polypeptide having ferulic acid esterase activity and comprising an amino acid sequence having at least 90% sequence identity to an amino acid sequence of SEQ ID NO: 11 or SEQ ID NO: 12 in a spent culture medium, wherein the enzyme preparation further comprising additives.

### Claim 2 (depends on 1)

The detergent composition according to claim 1, wherein the composition is a dishwashing machine cleaning composition.