# US 9,434,923 B2 · Preparation of parental cell bank from foetal tissue > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,434,923 B2 - Document ID: US9434923B2 - Status: Granted - Kind: B2 - Application number: 14/620,321 - Filed: 2015-02-12 - Published: 2015-06-04 - Granted: 2016-09-06 - Earliest claimed: 2011-07-11 - Assignees: Centre Hospitalier Universitaire Vaudois (Chuv) - Applicants: Centre Hospitalier Universitaire Vaudois (Chuv) - Inventors: Lee Ann Laurent-Applegate - Web page: https://patentgrep.com/patent/US9434923B2 ## CPC - C12N 5/0655: {Chondrocytes; Cartilage} - A61K 35/32: Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane - A61K 35/33: Fibroblasts - A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like - A61P 19/04: for non-specific disorders of the connective tissue - A61P 19/08: for bone diseases, e.g. rachitism, Paget's disease - C12N 5/0603: {Embryonic cells (production of embryos, nuclear transfer A01K67/027); Embryoid bodies} - C12N 5/066: {Tenocytes; Tendons, Ligaments} - G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics} - G01N 2500/10: involving cells - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair. ## Claims ### Claim 1 A scaffold comprising of a biocompatible, non-living material having an interstitial space inoculated with a foetal epiphyseal chondrocyte (FEC) cell belonging to a cell line having the designation FE002-Cart and deposited under accession number ECACC 12070303-FE002-Cart. ### Claim 2 A scaffold comprising of a biocompatible, non-living material having an interstitial space inoculated with a foetal Achilles tenocyte cell belonging to a cell line having the designation FE002-Ten and deposited under accession number ECACC 12070302-FE002-Ten. ### Claim 3 A scaffold comprising of a biocompatible, non-living material having an interstitial space inoculated with a foetal skin fibroblast cell belonging to a cell line having the designation FE002-SK2 and deposited under accession number ECACC 12070301-SK2. ### Claim 4 (depends on 1) A method of producing new cartilage tissue and/or three dimensional constructs, the method comprising culturing the FEC cell in the scaffold of claim 1. ### Claim 5 (depends on 2) A method of producing new tendon tissue and/or three dimensional constructs, the method comprising culturing the foetal Achilles tenocyte cell in the scaffold of claim 2. ### Claim 6 (depends on 3) A method of producing new skin tissue and/or three dimensional constructs, the method comprising culturing the foetal skin fibroblast cell in the scaffold of claim 3. ### Claim 7 (depends on 1) A method for repair and regeneration of osteochondral tissue and musculoskeletal tissue, the method comprising administering the scaffold of claim 1 to a subject in need thereof. ### Claim 8 (depends on 2) A method for repair and regeneration of tendon tissue and musculoskeletal tissue, the method comprising administering the scaffold of claim 2 to a subject in need thereof. ### Claim 9 (depends on 3) A method for repair and regeneration of skin tissue and for treating burns, wounds and fibrotic conditions, the method comprising administering the scaffold of claim 3 to a subject in need thereof. ### Claim 10 (depends on 1) A method for treating osteochondral diseases or defects, arthritis and musculoskeletal diseases, the method comprising administering the scaffold of claim 1 to a subject in need thereof. ### Claim 11 (depends on 2) A method for treating musculoskeletal diseases and tendonopathies, the method comprising administering the scaffold of claim 2 to a subject in need thereof. ### Claim 12 (depends on 3) A method for treating skin diseases, the method comprising administering the scaffold of claim 3 to a subject in need thereof. ### Claim 13 (depends on 1) A screening method for development of a therapeutic agent for the treatment of arthritic, osteochondral detects or musculoskeletal tissue injury, the method comprising exposing the scaffold of claim 1 to the therapeutic agent. ### Claim 14 (depends on 2) A screening method for development of a therapeutic agent for the treatment of cartilage, tendon, or musculoskeletal tissue injury, the method comprising exposing the scaffold of claim 2 to the therapeutic agent. ### Claim 15 (depends on 3) A screening method for development of a therapeutic agent for the treatment of skin injury, the method comprising exposing the scaffold of claim 3 to the therapeutic agent. ### Claim 16 (depends on 1) The scaffold of claim 1, wherein the scaffold is: (a) a three dimensional matrix composed of one or materials selected from the group consisting of collagen, polylactic acid, polyglycolic acid, polyethylene glycol, chitosan, and elastin; or (b) a hydrogel composed of one or more materials selected from the group consisting of hyaluronic acid, silicone, and chitosan. ### Claim 17 (depends on 2) The scaffold of claim 2, wherein the scaffold is: (a) a three dimensional matrix composed of one or materials selected from the group consisting of collagen, polylactic acid, polyglycolic acid, polyethylene glycol, chitosan, and elastin; or (b) a hydrogel composed of one or more materials selected from the group consisting of hyaluronic acid, silicone, and chitosan. ### Claim 18 (depends on 3) The scaffold of claim 3, wherein the scaffold is: (a) a three dimensional matrix composed of one or materials selected from the group consisting of collagen, polylactic acid, polyglycolic acid, polyethylene glycol, chitosan, and elastin; or (b) a hydrogel composed of one or more materials selected from the group consisting of hyaluronic acid, silicone, and chitosan.