# US 9,434,915 B2 · Compositions and methods comprising a subtilisin variant

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- Number: US 9,434,915 B2
- Document ID: US9434915B2
- Status: Granted
- Kind: B2
- Application number: 14/615,299
- Filed: 2015-02-05
- Published: 2015-08-27
- Granted: 2016-09-06
- Earliest claimed: 2008-11-11
- Assignees: Danisco US Inc.
- Applicants: Danisco US Inc.
- Inventors: David A. Estell; Frits Goedegebuur; Ayrookaran J. Poulose
- Web page: https://patentgrep.com/patent/US9434915B2

## CPC

- C11D 3/386: Preparations containing enzymes {, e.g. protease or amylase}
- C11D 3/38609: {Protease or amylase in solid compositions only}
- C11D 3/38618: {Protease or amylase in liquid compositions only}
- C11D 3/38681: {Chemically modified or immobilised enzymes}
- C11D 3/3942: {Inorganic per-compounds (C11D3/3902 takes precedence)}
- C12N 9/54: bacteria being Bacillus
- C12Y 304/21062: Subtilisin (3.4.21.62)
- C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12Y: ENZYMES

## Abstract

The present invention provides a Bacillus sp. subtilisin variant. In addition, the present invention provides automatic dishwashing compositions comprising this serine protease variant.

## Claims

### Claim 1

A subtilisin variant comprising the amino acid sequence set forth in SEQ ID NO:8.

### Claim 2 (depends on 1)

An automatic dishwashing detergent composition comprising the subtilisin variant of claim 1.

### Claim 3 (depends on 2)

The automatic dishwashing detergent composition of claim 2, wherein said composition is a liquid detergent.

### Claim 4 (depends on 2)

The automatic dishwashing detergent composition of claim 2, wherein said composition is a powder, tablet, gel or granule detergent.

### Claim 5 (depends on 2)

The automatic dishwashing detergent composition of claim 2, wherein said composition does not contain phosphate.

### Claim 6 (depends on 2)

The automatic dishwashing detergent composition of claim 2, further comprising at least one bleaching agent.

### Claim 7 (depends on 2)

The automatic dishwashing detergent composition of claim 2, further comprising at least one additional enzyme.

### Claim 8 (depends on 7)

The automatic dishwashing detergent composition of claim 7, wherein said at least one additional enzyme is selected from hemicellulases, cellulases, peroxidases, proteases, metalloproteases, xylanases, lipases, phospholipases, esterases, perhydrolases, cutinases, pectinases, pectate lyases, mannanases, keratinases, reductases, oxidases, phenoloxidases, lipoxygenases, ligninases, pullulanases, tannases, pentosanases, malanases, β-glucanases, arabinosidases, hyaluronidase, chondroitinase, laccase, and amylases, or mixtures thereof.

### Claim 9

A method for cleaning comprising providing a dishware item to be cleaned and a composition comprising the subtilisin variant having the amino acid sequence set forth in SEQ ID NO:8, and contacting said item or surface with said composition.

### Claim 10 (depends on 9)

The method of claim 9, further comprising the step of rinsing said dishware item to be cleaned.

### Claim 11 (depends on 6)

The automatic dishwashing detergent composition of claim 6, wherein the bleaching agent is selected from inorganic bleaching compound, organic bleaching compound, and bleach activator.

### Claim 12 (depends on 11)

The automatic dishwashing detergent composition of claim 11, wherein the inorganic bleaching compound is selected from perborates, percarbonates, perphosphates, persulfates, and persilicates.

### Claim 13 (depends on 11)

The automatic dishwashing detergent composition of claim 11, wherein the bleach activator is selected from organic peracids.

### Claim 14 (depends on 2)

The automatic dishwashing detergent composition of claim 2, further comprising at least one builder.

### Claim 15 (depends on 2)

The automatic dishwashing detergent composition of claim 2, further comprising a metal care agent.