# US 9,433,922 B2 · Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same

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- Number: US 9,433,922 B2
- Document ID: US9433922B2
- Status: Granted
- Kind: B2
- Application number: 12/221,496
- Filed: 2008-08-04
- Published: 2009-02-26
- Granted: 2016-09-06
- Earliest claimed: 2007-08-14
- Assignees: EMD Millipore Corporation
- Applicants: Mikhail Kozlov; Wilson Moya; Michael W. Phillips; Senthilkumar Ramaswamy; Brian Gagnon
- Inventors: Mikhail Kozlov; Wilson Moya; Michael W. Phillips; Senthilkumar Ramaswamy; Brian Gagnon
- Web page: https://patentgrep.com/patent/US9433922B2

## CPC

- B01J 20/28033: {Membrane, sheet, cloth, pad, lamellar or mat}
- B01D 15/1871: {placed in series}
- B01D 15/1896: {Membrane chromatography or membrane adsorbers}
- B01D 15/362: {Cation-exchange}
- B01D 15/363: {Anion-exchange}
- B01D 15/3804: {Affinity chromatography}
- B01J 20/3282: {Crosslinked polymers}
- B01J 47/12: characterised by the use of ion-exchange material in the form of ribbons, filaments, fibres or sheets, e.g. membranes (electrodialysis or electro-osmosis B01D61/42)
- Y10T 428/249987: With nonvoid component of specified composition
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01D: SEPARATION (separating solids from solids by wet methods B03B, B03D; by pneumatic jigs or tables B03B; by other dry methods B07; magnetic or electrostatic separation of solid materials from solid materials or fluids, separation by high-voltage electric fields B03C; centrifuges, vortex apparatus B04; presses per se for squeezing-out liquid from liquid-containing material B30B9/02; treatment of water C02F, e.g. softening by ion-exchange C02F1/42; {arrangements of air intake cleaners in gas turbine plants F02C7/05} ; arrangements or mounting of filters in air-conditioning, air-humidification or ventilation F24F13/28)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

## Claims

### Claim 1

A porous sorptive media comprising a substrate having a first external side and a second external side, both sides being porous, and a porous thickness between them, said substrate having a sorptive material substantially covering the solid matrix of the substrate and said first and second external surfaces, said sorptive material comprising protonated polyallylamine crosslinked with polyethylene glycol diglycidyl ether, wherein said crosslinked protonated polyallylamine is present in an amount effective to produce a binding capacity of about 60 g/L BSA binding at 200 mM NaCl, and the thickness of sorptive material substantially covering said first and second external surfaces is 10-50 nm.

### Claim 2 (depends on 1)

The porous sorptive media of claim 1, wherein said substrate comprises a microporous membrane.

### Claim 3 (depends on 2)

The porous sorptive media of claim 2, wherein said membrane comprises a polyolefin.

### Claim 4 (depends on 3)

The porous sorptive media of claim 3, wherein said polyolefin is polyethylene.

### Claim 5 (depends on 1)

The porous sorptive media of claim 1, wherein said substrate is an ultrahigh molecular weight polyethylene membrane.

### Claim 6 (depends on 1)

The porous sorptive media of claim 1, having a nucleic acid binding capacity of greater than 5 mg/ml.

### Claim 7 (depends on 6)

The porous sorptive media of claim 6, wherein said nucleic acid is DNA.

### Claim 8 (depends on 1)

The porous sorptive media of claim 1, wherein said substrate comprises a non-woven material.

### Claim 9 (depends on 8)

The porous sorptive media of claim 8, wherein said non-woven material is a mat or fabric.