# US 9,433,727 B1 · Tissue rejuvenation methods and tissue transfer devices suitable for implementation thereof > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,433,727 B1 - Document ID: US9433727B1 - Status: Granted - Kind: B1 - Application number: 14/173,021 - Filed: 2014-02-05 - Granted: 2016-09-06 - Earliest claimed: 2013-02-05 - Applicants: Simeon Wall, Jr. - Inventors: Simeon Wall, Jr. - Web page: https://patentgrep.com/patent/US9433727B1 ## CPC - A61M 1/895: {with means for reinjection of collected fat} - A61B 17/22: Implements for squeezing-off ulcers or the like on inner organs of the body; Implements for scraping-out cavities of body organs, e.g. bones; for invasive removal or destruction of calculus using mechanical vibrations; for removing obstructions in blood vessels, not otherwise provided for - A61M 1/77: {Suction-irrigation systems (aspiration tips supplying fluids A61M1/85; specific for negative pressure wound therapy A61M1/92; combined with tracheal tubes A61M16/0463)} - A61M 5/14212: {Pumping with an aspiration and an expulsion action} - A61M 25/0074: {Dynamic characteristics of the catheter tip, e.g. openable, closable, expandable or deformable} - A61B 2017/00424: {ergonomic, e.g. fitting in fist} - A61B 2017/00792: {Plastic surgery} - A61B 2017/00969: {used for transplantation} - A61M 25/007: {Side holes, e.g. their profiles or arrangements; Provisions to keep side holes unblocked} - A61M 2025/0004: {having two or more concentrically arranged tubes for forming a concentric catheter system} - A61M 2025/1047: {having centering means, e.g. balloons having an appropriate shape} - A61M 2202/08: Lipoids - A61M 2202/10: Bone-marrow - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) ## Abstract Tissue rejuvenation methods include identifying a cell harvest site on a patient, identifying a cell injection site on the patient, harvesting tissue regenerative cells from the cell harvest site, agitating the tissue regenerative cells and injecting the tissue regenerative cells into the cell injection site. Tissue transfer devices suitable for implementation of the tissue rejuvenation methods are also disclosed. ## Claims ### Claim 1 A tissue rejuvenation method, comprising: identifying a cell harvest site on a patient; identifying a cell injection site on the patient; harvesting tissue regenerative cells from the cell harvest site by providing a tissue transfer device having a cannula and tissue separating members outwardly extendable from the cannula, inserting the cannula in the cell harvest site, separating portions of harvest site adipose tissue from each other by expanding or buckling the tissue separating members outwardly from the cannula in a curved or bowed configuration against the harvest site adipose tissue and drawing the tissue regenerative cells from the cell harvest site through the cannula, the tissue regenerative cells including at least one of the following: adipose-derived stromal cells, pericytes and stem cells from bone marrow; agitating the tissue regenerative cells; and injecting the tissue regenerative cells into the cell injection site. ### Claim 2 (depends on 1) The tissue rejuvenation method of claim 1 wherein harvesting tissue regenerative cells from the cell harvest site comprises harvesting pericytes from the cell harvest site. ### Claim 3 (depends on 1) The tissue rejuvenation method of claim 1 wherein harvesting tissue regenerative cells from the cell harvest site comprises harvesting adipose-derived stromal cells from the cell harvest site. ### Claim 4 (depends on 1) The tissue rejuvenation method of claim 1 wherein agitating the tissue regenerative cells comprises mechanically agitating the tissue regenerative cells while injecting the tissue regenerative cells into the cell injection site. ### Claim 5 (depends on 1) The tissue rejuvenation method of claim 1 wherein harvesting tissue regenerative cells from the cell harvest site comprises separating portions of harvest site adipose tissue from each other. ### Claim 6 (depends on 1) The tissue rejuvenation method of claim 1 wherein agitating the tissue regenerative cells comprises mechanically agitating the tissue regenerative cells by repeated bidirectional movement of the tissue transfer device while injecting the tissue regenerative cells into the cell injection site. ### Claim 7 (depends on 1) The tissue rejuvenation method of claim 1 wherein injecting the tissue regenerative cells into the cell injection site comprises separating portions of harvest site adipose tissue from each other. ### Claim 8 A tissue rejuvenation method, comprising: identifying and marking a cell harvest site on a patient; identifying and marking a cell injection site on the patient; providing a tissue transfer device including a cannula and tissue separating members outwardly extendable from the cannula; harvesting tissue regenerative cells from the cell harvest site by inserting the cannula of the tissue transfer device into the cell harvest site, expanding or buckling the tissue separating members outwardly from the cannula in a curved or bowed configuration against the harvest site adipose tissue and aspirating the tissue regenerative cells from the cell harvest site through the cannula, the tissue regenerative cells including at least one of the following: adipose-derived stem cells, pericytes and stem cells from bone marrow; agitating the tissue regenerative cells; and injecting the tissue regenerative cells into the cell injection site by inserting the cannula of the tissue transfer device into the cell injection site and injecting the tissue regenerative cells through the cannula into the cell injection site. ### Claim 9 (depends on 8) The tissue rejuvenation method of claim 8 wherein harvesting tissue regenerative cells from the cell harvest site comprises harvesting pericytes from the cell harvest site. ### Claim 10 (depends on 8) The tissue rejuvenation method of claim 8 wherein harvesting tissue regenerative cells from the cell harvest site comprises harvesting adipose-derived stromal cells from the cell harvest site. ### Claim 11 (depends on 8) The tissue rejuvenation method of claim 8 wherein agitating the tissue regenerative cells comprises mechanically agitating the tissue regenerative cells while injecting the tissue regenerative cells into the cell injection site. ### Claim 12 (depends on 8) The tissue rejuvenation method of claim 8 wherein mechanically agitating the tissue regenerative cells comprises repeated bidirectional movement of the tissue transfer device while injecting the tissue regenerative cells into the cell injection site. ### Claim 13 (depends on 8) The tissue rejuvenation method of claim 8 wherein injecting the tissue regenerative cells into the cell injection site comprises separating portions of harvest site adipose tissue from each other. ### Claim 14 A tissue rejuvenation method, comprising: identifying and marking a cell harvest site on a patient; identifying and marking a cell injection site on the patient; providing a tissue transfer device including a cannula, tissue separating members outwardly extendable from the cannula, a tissue reservoir disposed in fluid communication with the cannula and a pump disposed in fluid communication with the tissue reservoir; harvesting tissue regenerative cells from the cell harvest site by inserting the cannula of the tissue transfer device into the cell harvest site, separating portions of harvest site adipose tissue from each other by expanding or buckling the tissue separating members outwardly from the cannula in a curved or bowed configuration against the harvest site adipose tissue, aspirating the tissue regenerative cells from the cell harvest site through the cannula and into the tissue reservoir by operation of the pump and retracting the tissue separating members, the tissue regenerative cells including at least one of the following: adipose-derived stem cells, pericytes and stem cells from bone marrow; agitating the tissue regenerative cells; and injecting the tissue regenerative cells into the cell injection site by inserting the cannula of the tissue transfer device into the cell injection site, separating portions of injection site adipose tissue from each other by expanding the tissue separating members outwardly from the cannula against the injection site adipose tissue and injecting the tissue regenerative cells from the tissue reservoir through the cannula into the cell injection site by operation of the pump. ### Claim 15 (depends on 14) The tissue rejuvenation method of claim 14 wherein harvesting tissue regenerative cells from the cell harvest site comprises harvesting pericytes from the cell harvest site. ### Claim 16 (depends on 14) The tissue rejuvenation method of claim 14 wherein harvesting tissue regenerative cells from the cell harvest site comprises harvesting adipose-derived stromal cells from the cell harvest site. ### Claim 17 (depends on 14) The tissue rejuvenation method of claim 14 wherein agitating the tissue regenerative cells comprises mechanically agitating the tissue regenerative cells while injecting the tissue regenerative cells into the cell injection site. ### Claim 18 (depends on 14) The tissue rejuvenation method of claim 14 wherein mechanically agitating the tissue regenerative cells comprises repeated bidirectional movement of the tissue transfer device while injecting the tissue regenerative cells into the cell injection site.