# US 9,433,218 B2 · Isolated bacterial strain of the genus Burkholderia and pesticidal metabolites therefrom

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 9,433,218 B2
- Document ID: US9433218B2
- Status: Granted
- Kind: B2
- Application number: 14/336,601
- Filed: 2014-07-21
- Published: 2014-12-11
- Granted: 2016-09-06
- Earliest claimed: 2010-02-25
- Assignees: Marrone Bio Innovations, Inc.
- Applicants: Marrone Bio Innovations, Inc.
- Inventors: Ratnakar Asolkar; Marja Koivunen; Pamela Marrone; Ana Lucia Cordova-Kreylos; Huazhang Huang
- Web page: https://patentgrep.com/patent/US9433218B2

## CPC

- A01N 63/20: Bacteria; Substances produced thereby or obtained therefrom
- A01N 43/16: with oxygen as the ring hetero atom
- A01N 43/76: 1,3-Oxazoles; Hydrogenated 1,3-oxazoles
- A01N 43/86: six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
- A01N 43/90: having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
- A01N 57/20: containing acyclic or cycloaliphatic radicals
- C07D 263/32: with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
- C07D 263/34: with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D 309/14: Nitrogen atoms not forming part of a nitro radical
- C07D 407/06: linked by a carbon chain containing only aliphatic carbon atoms
- C07D 413/04: directly linked by a ring-member-to-ring-member bond
- C07D 413/06: linked by a carbon chain containing only aliphatic carbon atoms
- C07D 493/10: Spiro-condensed systems
- C07D 498/14: Ortho-condensed systems
- C07D 513/04: Ortho-condensed systems
- C12N 1/20: Bacteria; Culture media therefor
- C12N 1/205: Bacterial isolates
- C12P 17/04: containing a five-membered hetero ring, e.g. griseofulvin {, vitamin C}
- C12P 17/06: containing a six-membered hetero ring, e.g. fluorescein
- C12P 17/14: Nitrogen or oxygen as hetero atom and at least one other diverse hetero ring atom in the same ring
- C12P 17/16: containing two or more hetero rings {(thiamine open chain analogs C12P17/167, i.e. not condensed among themselves or through a common carbocyclic ring system)}
- C12P 17/18: containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin {(e.g. Rifamycin C12P17/189)}
- A01N 43/00: Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds (containing cyclic anhydrides, cyclic imides A01N37/00; containing compounds of the formula [IMAGE cpc-sch-A01N-0942.gif] containing only one heterocyclic ring, wherein m>=1 and n>=0 and [IMAGE cpc-sch-A01N-0943.gif]is unsubstituted or alkylsubstituted pyrrolidine, piperidine, morpholine, thiomorpholine, piperazine or a polymethyleneimine with four or more CH2 groups, A01N33/00 - A01N41/12; containing cyclopropane carboxylic acids or derivatives thereof, e.g. esters having heterocyclic rings, A01N53/00)
- C12N 1/00: Microorganisms; Compositions thereof (medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00); Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12R 2001/00: Microorganisms {; Processes using microorganisms}
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- A01N: PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF (preservation of food or foodstuff A23); BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES (preparations for medical, dental or toiletry purposes which kill or prevent the growth or proliferation of unwanted organisms A61K); PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- C07D: HETEROCYCLIC COMPOUNDS (macromolecular compounds C08)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, insects, fungi, weeds and nematodes) is provided. Also provided are natural products derived from a culture of said species and methods of controlling pests using said natural products.

## Claims

### Claim 1

A method for modulating pest infestation in a plant comprising the step of:
applying to the plant and/or seeds thereof and/or substrate used for growing said plant an effective amount of a fermented composition comprising whole cell broth from a Burkholderia strain A396 (NRRL Accession No. B-50319) fermentation, wherein said fermented composition comprises templamide A, templamide B, FR901465 and/or FR90128 to modulate said pest infestation.

### Claim 2 (depends on 1)

The method of claim 1, wherein said composition further comprises one or more chemical and/or biological pesticide.

### Claim 3 (depends on 1)

The method of claim 1, wherein said composition further comprises one or more carriers, diluent, surfactants, and/or adjuvants.

### Claim 4 (depends on 1)

The method of claim 1, wherein said pest comprises nematodes, beet armyworms, green peach aphids, cabbage loopers and/or stinkbugs.

### Claim 5 (depends on 4)

The method of claim 4, wherein said nematodes comprise root-knot, ring, sting, lance, cyst, or lesion nematodes.

### Claim 6 (depends on 1)

The method of claim 1, wherein said composition further comprises Templazole A, Templazole B or both.