# US 9,433,195 B2 · Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,433,195 B2 - Document ID: US9433195B2 - Status: Granted - Kind: B2 - Application number: 13/840,598 - Filed: 2013-03-15 - Published: 2013-12-12 - Granted: 2016-09-06 - Earliest claimed: 2012-06-06 - Assignees: Inguran, LLC - Applicants: Inguran, LLC - Inventors: Juan Moreno; Gregg BeVier; John Dobrinsky - Web page: https://patentgrep.com/patent/US9433195B2 ## CPC - A01K 67/02: Breeding vertebrates - A61B 1/015: Control of fluid supply or evacuation - A61B 1/04: combined with photographic or television appliances - A61B 1/303: for the vagina, i.e. vaginoscopes - A61B 1/313: for introducing through surgical openings, e.g. laparoscopes - A61B 8/08: Clinical applications (A61B8/02, A61B8/04, A61B8/06 take precedence) - A61B 10/0012: {Ovulation-period determination (for animals A61D17/002)} - A61B 34/20: Surgical navigation systems; Devices for tracking or guiding surgical instruments, e.g. for frameless stereotaxis - A61D 19/027: {Devices for injecting semen into animals, e.g. syringes, guns, probes} - A61M 31/002: {Devices for releasing a drug at a continuous and controlled rate for a prolonged period of time (artificial gland structures or devices A61F2/022; intra-uterine contraceptive devices A61F6/14; tampons for introducing into the vagina A61F13/20, A61L15/00; suppositories or bougies for intra-vaginal or intra-uterine application A61K9/02; physical forms of medicinal preparations for sustained or differential drug release A61K9/20, A61K9/50)} - G16B 20/40: Population genetics; Linkage disequilibrium - A01K 2227/108: Swine - A61B 2034/2057: {Details of tracking cameras} - G16B 20/00: ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations - A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - A61D: VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS - A61M: DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY (introducing media into or onto the bodies of animals A61D7/00; means for inserting tampons A61F13/26; devices for administering food or medicines orally A61J; containers for collecting, storing or administering blood or medical fluids A61J1/05); DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY (surgery A61B; chemical aspects of surgical articles A61L); DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR {(Electrotherapy, e.g. producing anaesthesia by the use of alternating or intermittent currents A61N1/36021)} - G16B: BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY ## Abstract The invention relates to methods of increasing the genetic progress of a line, breed or herd of swine through the use of sex-selected sperm cells in artificial insemination techniques. The invention also encompasses methods of artificially inseminating a swine via deep intrauterine catheter or via a laparoscopic procedure, which allow the use of reduced doses of sex-selected sperm cells. ## Claims ### Claim 1 A method of increasing the genetic progress of a line or breed of swine within a genetic nucleus comprising the steps of: providing a boar and a sow from a genetic nucleus, wherein said boar and said sow are from a line or breed within the genetic nucleus and wherein the line or breed of said boar and said sow are the same line or breed; collecting a semen sample from said boar; sorting said semen sample into at least two subpopulations of sperm cells, wherein at least 80% of a first subpopulation bears X-chromosomes or Y-chromosomes; inseminating the sow with sperm cells from said first subpopulation to produce offspring from said sow; providing a selection index; calculating the selection index for one or more of said offspring; calculating an average of the selection index for said line or breed within the genetic nucleus; and breeding said one or more of said offspring with other swine in the line or breed of said boar and said sow within the genetic nucleus when the selection index for one or more of said offspring is greater than the average of the selection index for said line or breed within the genetic nucleus, thereby increasing the genetic progress of a line or breed of swine in the genetic nucleus. ### Claim 2 (depends on 1) The method of claim 1, wherein said line or breed is a gilt line and said first subpopulation bears X-chromosomes. ### Claim 3 (depends on 1) The method of claim 1, wherein said line or breed is a boar line and said first subpopulation bears Y-chromosomes. ### Claim 4 (depends on 1) The method of claim 1, wherein the selection index for one or more of said offspring is calculated based on data derived from a group comprising said offspring. ### Claim 5 (depends on 1) The method of claim 1, wherein said selection index comprises traits for fertility, litter size and milk production. ### Claim 6 (depends on 1) The method of claim 1, wherein said selection index comprises traits for feed efficiency, average daily gain and lean carcass. ### Claim 7 (depends on 1) The method of claim 1, wherein the step of inseminating the sow comprises administering said sperm cells into the reproductive tract of said sow by deep intrauterine catheter or laparoscopy. ### Claim 8 (depends on 7) The method of claim 7, wherein administering said sperm cells to said sow's reproductive tract using a deep intrauterine catheter comprises placing said sperm cells in one or more uterine horns. ### Claim 9 (depends on 8) The method of claim 8, wherein said deep intrauterine catheter comprises a video camera or scope. ### Claim 10 (depends on 8) The method of claim 8, further comprising the step of visualizing the deep intrauterine catheter via radiography or fluoroscopy while inserted in said sow's reproductive tract. ### Claim 11 (depends on 8) The method of claim 8, wherein the total number of sperm cells administered is 50×106 or less sperm cells. ### Claim 12 (depends on 7) The method of claim 7, wherein administering said sperm cells to said sow's reproductive tract by laparoscopy comprises injecting said sperm cells into one or more oviducts of said sow's uterus. ### Claim 13 (depends on 12) The method of claim 12, wherein the total number of sperm cells administered is 1×106 or less sperm cells. ### Claim 14 (depends on 1) The method of claim 1, further comprising the step of inducing estrus in said sow by administering one or more hormone or hormone analog to said sow. ### Claim 15 (depends on 14) The method of claim 14, wherein said one or more hormone or hormone analog comprises PG600, OvuGel, eCG, progestin, or hCG. ### Claim 16 (depends on 14) The method of claim 14, wherein said one or more hormone or hormone analog is administered by a programmable device placed in the reproductive tract of said sow. ### Claim 17 (depends on 14) The method of claim 14, further comprising the step of detecting ovulation in said sow by examining said females follicles. ### Claim 18 (depends on 17) The method of claim 17, wherein said follicles are examined using ultrasound. ### Claim 19 A method of increasing the genetic progress of a line or breed of swine within a genetic nucleus comprising the steps of: providing a boar and a sow from a genetic nucleus, wherein said boar and said sow are from a line or breed within the genetic nucleus and wherein the line or breed of said boar and said sow are the same line or breed; collecting a semen sample from said boar; sorting said semen sample into at least two subpopulations of sperm cells, wherein at least 80% of a first subpopulation bears X chromosomes or Y chromosomes; inseminating said sow with sperm cells from said first subpopulation to produce offspring from said sow; identifying a trait; obtaining a value for the trait in one or more of said offspring; calculating an average value of the trait for said line or breed within the genetic nucleus; and breeding said one or more of said offspring with other swine in the line or breed of said boar and said sow within the genetic nucleus when the value of the trait for said offspring is greater than or less than the average value for the trait for said line or breed within the genetic nucleus, thereby increasing the genetic progress of a line or breed of swine in the genetic nucleus. ### Claim 20 (depends on 19) The method of claim 19, wherein said line or breed is a gilt line and said first subpopulation bears X-chromosomes. ### Claim 21 (depends on 19) The method of claim 19, wherein said line or breed is a boar line and said first subpopulation bears Y-chromosomes.