# US 9,433,162 B2 · Celery cultivar TBG32

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- Number: US 9,433,162 B2
- Document ID: US9433162B2
- Status: Granted
- Kind: B2
- Application number: 14/577,011
- Filed: 2014-12-19
- Published: 2016-06-23
- Granted: 2016-09-06
- Earliest claimed: 2014-12-19
- Assignees: A. Duda & Sons, Inc.
- Applicants: A. Duda & Sons, Inc.
- Inventors: Lawrence K. Pierce
- Web page: https://patentgrep.com/patent/US9433162B2

## CPC

- A01H 6/064: {Apium graveolens [celery]}
- A01H 5/04: Stems
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A celery cultivar, designated TBG32, is disclosed. The invention relates to the seeds of celery cultivar TBG32, to the plants of celery cultivar TBG32 and to methods for producing a celery plant by crossing the cultivar TBG32 with itself or another celery cultivar. The invention further relates to methods for producing a celery plant containing in its genetic material one or more transgenes and to the transgenic celery plants and plant parts produced by those methods. This invention also relates to celery cultivars or breeding cultivars and plant parts derived from celery cultivar TBG32, to methods for producing other celery cultivars, lines or plant parts derived from celery cultivar TBG32 and to the celery plants, varieties, and their parts derived from the use of those methods. The invention further relates to hybrid celery seeds, plants, and plant parts produced by crossing cultivar TBG32 with another celery cultivar.

## Claims

### Claim 1

A seed of celery cultivar TBG32, wherein a representative sample seed of said cultivar was deposited under ATCC Accession No. PTA-123108.

### Claim 2 (depends on 1)

A celery plant, or a part thereof, produced by growing the seed of claim 1.

### Claim 3 (depends on 2)

A tissue culture produced from protoplasts or cells from the plant of claim 2, wherein said cells or protoplasts are produced from a plant part selected from the group consisting of leaf, callus, pollen, ovule, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, pistil, anther, flower, seed, shoot, stem, petiole and sucker.

### Claim 4 (depends on 3)

A celery plant regenerated from the tissue culture of claim 3, wherein the plant has all of the morphological and physiological characteristics of cultivar TBG32.

### Claim 5 (depends on 2)

A method for producing a celery seed, said method comprising crossing two celery plants and harvesting the resultant celery seed, wherein at least one celery plant is the celery plant of claim 2.

### Claim 6 (depends on 5)

An F1 celery seed produced by the method of claim 5.

### Claim 7 (depends on 6)

An F1 celery plant, or a part thereof, produced by growing said seed of claim 6.

### Claim 8 (depends on 5)

The method of claim 5, wherein at least one of said celery plants is transgenic.

### Claim 9 (depends on 2)

A method of producing a herbicide resistant celery plant, wherein said method comprises introducing a gene conferring herbicide resistance into the plant of claim 2.

### Claim 10 (depends on 9)

A herbicide resistant celery plant produced by the method of claim 9, wherein the gene confers resistance to a herbicide selected from the group consisting of glyphosate, sulfonylurea, imidazolinone, dicamba, glufosinate, phenoxy proprionic acid, L-phosphinothricin, cyclohexone, cyclohexanedione, triazine, and benzonitrile, wherein said plant comprises said gene and otherwise comprises all of the physiological and morphological characteristics of celery cultivar TBG32.

### Claim 11 (depends on 2)

A method of producing a pest or insect resistant celery plant, wherein said method comprises introducing a gene conferring pest or insect resistance into the celery plant of claim 2.

### Claim 12 (depends on 11)

A pest or insect resistant celery plant produced by the method of claim 11, wherein said plant comprises said gene and otherwise comprises all of the physiological and morphological characteristics of celery cultivar TBG32.

### Claim 13 (depends on 12)

The celery plant of claim 12, wherein the gene encodes a Bacillus thuringiensis (Bt) endotoxin, wherein said plant comprises said gene and otherwise comprises all of the physiological and morphological characteristics of celery cultivar TBG32.

### Claim 14 (depends on 2)

A method of producing a disease resistant celery plant, wherein said method comprises introducing a gene into the celery plant of claim 2.

### Claim 15 (depends on 14)

A disease resistant celery plant produced by the method of claim 14, wherein said plant comprises said gene and otherwise comprises all of the physiological and morphological characteristics of celery cultivar TBG32.

### Claim 16 (depends on 2)

A method for producing a male sterile celery plant, wherein said method comprises transforming the celery plant of claim 2 with a nucleic acid molecule that confers male sterility.

### Claim 17 (depends on 16)

A male sterile celery plant produced by the method of claim 16, wherein said plant comprises said nucleic acid and otherwise comprises all of the physiological and morphological characteristics of celery cultivar TBG32.

### Claim 18

A method of introducing a desired trait into celery cultivar TBG32, wherein the method comprises:
(a) crossing a TBG32 plant, wherein a representative sample of seed was deposited under ATCC Accession No. PTA-123108, with a plant of another celery cultivar that comprises a desired trait to produce progeny plants, wherein the desired trait is selected from the group consisting of improved nutritional quality, industrial usage, male sterility, herbicide resistance, insect resistance, modified seed yield, modified lodging resistance, modified iron-deficiency chlorosis and resistance to bacterial disease, fungal disease or viral disease;
(b) selecting one or more progeny plants that have the desired trait to produce selected progeny plants;
(c) backcrossing the selected progeny plants with the TBG32 plants to produce backcross progeny plants;
(d) selecting for backcross progeny plants that have the desired trait; and
(c) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that comprise the desired trait.

### Claim 19 (depends on 18)

A celery plant produced by the method of claim 18, wherein the plant has the desired trait and otherwise all of the physiological and morphological characteristics of celery cultivar TBG32.

### Claim 20 (depends on 19)

The celery plant of claim 19, wherein the desired trait is herbicide resistance and the resistance is conferred to a herbicide selected from the group consisting of imidazolinone, dicamba, cyclohexanedione, sulfonylurea, glyphosate, glufosinate, phenoxy proprionic acid, L-phosphinothricin, triazine and benzonitrile.

### Claim 21 (depends on 19)

The celery plant of claim 19, wherein the desired trait is insect resistance and the insect resistance is conferred by a gene encoding a Bacillus thuringiensis endotoxin.

### Claim 22 (depends on 19)

The celery plant of claim 19, wherein the desired trait is male sterility and the trait is conferred by a cytoplasmic nucleic acid molecule.

### Claim 23 (depends on 2)

A method of producing a celery plant with modified fatty acid metabolism or modified carbohydrate metabolism comprising transforming the celery plant of claim 2 with a transgene encoding a protein selected from the group consisting of fructosyltransferase, levansucrase, α-amylase, invertase and starch branching enzyme or DNA encoding an antisense of stearyl-ACP desaturase.

### Claim 24 (depends on 23)

A celery plant having modified fatty acid metabolism or modified carbohydrate metabolism produced by the method of claim 23, wherein said plant comprises said transgene and otherwise comprises all of the physiological and morphological characteristics of celery cultivar TBG32.