Complex formation method and separation method
AssigneeWako Pure Chemical Industries, Ltd.
InventorsTomohisa Kawabata, Shinji Satomura, Henry Garrett Wada
Life of this patent
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Abstract
An object of the present invention is to provide a method for forming a complex between an analyte or an analog thereof, and a substance formable the complex with said analyte or said analog thereof (the complex forming substance), in a short time and in high reaction efficiency, and a method for separating a complex formed, and a complex forming substance not involved in formation of said complex or an analog not involved in formation of said complex rapidly, simply and in high accuracy, along with a method for measuring an analyte in a sample in high sensitivity.
Claims19
A method for separating a complex comprising the following steps:
- (1) a step of arranging (a) a solution containing an analyte or an analogue thereof and (b) a solution containing not less than one kind of a substance capable of forming the complex with said analyte or said analogue thereof (the complex forming substance), each of (a) and (b) in a separate zone in a capillary, so that by applying a voltage to said capillary the complex between said analyte or said analogue thereof and the complex forming substance are formed without mixing these solutions in advance;
- (2) a step of forming the complex by electrophoretically concentrating said analyte or said analogue thereof and/or the complex forming substance by utilizing a difference in their electrophoretic mobility wherein said analyte or said analogue thereof and/or said complex forming substance are gathered on application of a voltage onto a capillary by applying a voltage to said capillary, before these solutions are uniformly mixed to form the complex between said analyte or said analogue thereof and the complex forming substance;
- and
- (3) a step of separating said complex, and the complex forming substance not involved in the formation of said complex or the analogue not involved in the formation of said complex by further electrical movement by using a different principle of electrophoresis from that in step (2),
- wherein step (2) is carried out by ITP (isotachophoresis) or FASS (Field Amplification Sample Stacking).
A method for separating a complex comprising the following steps:
- (1) a step of arranging (a) a solution containing an analyte or an analogue thereof and (b) a solution containing not less than one kind of a substance capable of forming the complex with said analyte or said analogue thereof (the complex forming substance), each of (a) and (b) in a separate zone in a capillary, so that by applying a voltage to said capillary the complex between said analyte or said analogue thereof and the complex forming substance are formed without mixing these solutions in advance;
- (2) a step of forming the complex by electrophoretically concentrating said analyte or said analogue thereof and/or the complex forming substance by utilizing a difference in their electrophoretic mobility wherein said analyte or said analogue thereof and/or said complex forming substance are gathered on application of a voltage onto a capillary by applying a voltage to said capillary, before these solutions are uniformly mixed to form the complex between said analyte or said analogue thereof and the complex forming substance;
- (3) a step of separating said complex, and the complex forming substance not involved in the formation of said complex or the analogue not involved in the formation of said complex by further electrical movement by using a different principle of electrophoresis from that in step (2);
- and
- (4) a step of measuring the amount of thus separated complex, and/or the amount of the complex forming substance and/or the analogue not involved in the formation of said complex to determine the amount of said analyte based on the result,
- wherein step (2) is carried out by ITP (isotachophoresis) or FASS (Field Amplification Sample Stacking).
Dependencies are inferred from the claim text.