US 9,429,547 B1 · Systems and methods for automated preparation of nucleic acids

Abstract

A fabrication process for producing, annealing, and conjugating nucleic acid molecules is implemented, under the direction of one or more computer programs, on two or more instruments, for carrying out the process. The process can include: (a) synthesizing, on a solid support and in a plurality of reactions, at least one of a nucleic acid and a peptide nucleic acid, and cleaving the synthesized biomolecule product from the support, providing a sample of synthesized biomolecule product from each reaction of the plurality; (b) measuring, for each sample, the volume of the sample and/or the concentration of the product; (c) subjecting the product from each reaction to chromatography under conditions suitable for achieving single-base-resolution for the product, the conditions being a function of the volume and/or concentration measured in (b); and then (d) collecting and pooling, from the chromatography of (c) for each sample, peaks that correspond to the product.

Claims19

Dependent claims

A system for preparing a chimeric oligonucleotide, comprising at least two instruments and program code which, when executed, configures the system to:

(a) synthesize a first nucleic acid (NA) oligonucleotide and a second peptide nucleic acid (PNA) oligonucleotide, thereby providing a first sample of the first oligonucleotide and a second sample of the second oligonucleotide;
(b) determine the volume of each sample, the concentration of the oligonucleotide in each sample and whether each oligonucleotide has a secondary structure;
(c) subject each oligonucleotide to chromatography under conditions suitable for disrupting the secondary structure of the oligonucleotide if the oligonucleotide has a secondary structure, and achieving single base-resolution for each oligonucleotide, wherein the conditions are a function of the volume and/or concentration measured in step (b);
(d) collect each oligonucleotide from the chromatography;
and
(e) conjugate the first oligonucleotide to the second oligonucleotide to form a chimeric oligonucleotide.

3 more elements

Dependencies are inferred from the claim text.

Systems and methods for automated preparation of nucleic acids

Life of this patent

Systems and methods for automated preparation of nucleic acids

Life of this patent

Abstract

Claims19