# US 9,429,539 B2 · Biosensor using impedimetric real-time monitoring

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- Number: US 9,429,539 B2
- Document ID: US9429539B2
- Status: Granted
- Kind: B2
- Application number: 14/567,792
- Filed: 2014-12-11
- Published: 2015-08-06
- Granted: 2016-08-30
- Earliest claimed: 2010-12-08
- Assignees: Imec; Universiteit Hasselt
- Applicants: Imec; Universiteit Hasselt
- Inventors: Bart Van Grinsven; Ward De Ceuninck; Patrick Wagner
- Web page: https://patentgrep.com/patent/US9429539B2

## CPC

- G01N 27/327: Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements}
- B01L 3/50273: {characterised by the means or forces applied to move the fluids}
- B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)}
- C12Q 1/6825: Nucleic acid detection involving sensors
- C12Q 1/6827: for detection of mutation or polymorphism
- C12Q 1/6832: Enhancement of hybridisation reaction
- G01N 25/4873: {for a flowing, e.g. gas sample}
- G01N 27/021: {before and after chemical transformation of the material}
- G01N 27/3275: {Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction}
- G05B 15/02: electric
- B01L 2300/023: Sending and receiving of information, e.g. using Bluetooth®
- B01L 2300/0636: Integrated biosensor, microarrays
- Y10T 436/11: Automated chemical analysis
- Y10T 436/143333: Saccharide [e.g., DNA, etc.]
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- G05B: CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS (systems for controlling or regulating non-electric variables G05D; systems for regulating electric or magnetic variables G05F; control devices or systems insofar as characterised by mechanical features only G05G)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

A method an system is disclosed for the detection and/or allocation of at least one point mutation in target DNA and/or RNA duplexes. The method comprises obtaining a functionalized surface which is coated with probe DNA and/or RNA whereto target DNA and/or RNA duplexes are attached, contacting said functionalized surface to an electrolytic solution having a neutral pH in a flow cell and measuring a first impedance value within said electrolytic solution, and then adding a chemical to the electrolytic solution which is able to achieve denaturation of the target DNA and/or RNA. The method further comprises measuring a second impedance value within the flow cell after completion of the denaturation of the DNA and/or RNA target, and then obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution. The amount and/or allocation of point mutation(s) within the target DNA and/or RNA is then determined by calculating the denaturation-time constant based on the difference between the first and second impedance value and taking into account the impact of the chemical by third impedance value.

## Claims

### Claim 1

A bio-sensing device suitable for the detection of target DNA and/or RNA and/or the characterization (allocation) of point mutations in target DNA and/or RNA, said bio-sensing device comprising at least:
a flow cell equipped with an impedimetric analyzer,
a biocompatible and inert substrate disposed against the flow cell, such that at least one surface of said substrate is exposed to liquid flow within the flow cell, said substrate having covalently bound probe DNA and/or RNA on its at least one exposed surface, said probe DNA and/or RNA being configured to bind the target DNA and/or RNA,
a pumping system and switching valve connected to said flow cell,
a first liquid supply comprising a electrolytic solution connected to said pumping system and to said switching valve,
a second liquid supply comprising a chemical that is able to achieve denaturation of DNA and/or RNA such that any of the target DNA and/or RNA that is bound to the probe DNA and/or RNA is released from the probe DNA and/or RNA when exposed to the chemical, the second liquid supply being connected to said pumping system and to said switching valve,
a means for obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution said means for obtaining the value representative for the impact of the chemical on the impedance of the electrolytic solution being operatively connected to the impedimetric analyzer, and
a means for determining the amount of the target DNA and/or RNA and/or an allocation of point mutation(s) within the target DNA and/or RNA by calculating the denaturation-time constant based on the difference between the first and second impedance value and corrected for the impact of the chemical by the value representative for such impact, said means for determining the amount and/or allocation being operatively connected to the means for obtaining the value representative for the impact of the chemical on the impedance of the electrolytic solution.

### Claim 2 (depends on 1)

A bio-sensing device according to claim 1, wherein said means for obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution is a controller programmed for controlling the impedance analyzer and the pumping system and switching valve for removing the chemical from the flow cell by adding electrolytic solution and then measuring a third impedance value within the flow cell to determine the impact of the chemical on the impedance of the electrolytic solution, the third impedance value being the value representative for the impact of the chemical on the impedance of the electrolytic solution.

### Claim 3 (depends on 2)

A bio-sensing device according to claim 2, wherein the controller is programmed for providing control signals to the bio-sensing device for:
contacting the exposed surface on which the probe DNA and/or RNA is bound to which target DNA and/or RNA are duplexed with an electrolytic solution having a neutral pH in a flow cell and measuring a first impedance value within said electrolytic solution, and then
adding the chemical to the electrolytic solution to achieve denaturation of the target DNA and/or RNA, and then
measuring a second impedance value within the flow cell after completion of the denaturation of the target DNA and/or RNA, and then
obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution, and then
determining the amount of target DNA and/or RNA and/or allocation of point mutation(s) within the target DNA and/or RNA by calculating the denaturation-time constant based on the difference between the first and second impedance value and corrected for the impact of the chemical by the value representative for such impact.

### Claim 4 (depends on 3)

A bio-sensing device according to claim 3, the controller being a computer program product.

### Claim 5 (depends on 1)

A bio-sensing device according to claim 1, wherein the flow cell has a volume between 25 μL and 190 μL.

### Claim 6 (depends on 1)

A bio-sensing device according to claim 1, wherein the substrate is nanocrystalline diamond, or a semiconductor substrate having a diamond coating 50-150 nm in thickness at its surface exposed to liquid flow within the flow cell.

### Claim 7 (depends on 1)

A bio-sensing device according to claim 1, wherein the probe DNA and/or RNA is NH2-modified single stranded DNA (ssDNA).

### Claim 8 (depends on 7)

A bio-sensing device according to claim 7, wherein the NH2-modified single stranded DNA is covalently bound to the surface exposed to liquid flow within the flow cell through a fatty acyl group.

### Claim 9 (depends on 1)

A bio-sensing device according to claim 1, wherein the chemical that is able to achieve denaturation of DNA and/or RNA is NaOH.

### Claim 10 (depends on 1)

A bio-sensing device according to claim 1, herein the flow cell has a volume between 25 μL and 190 μL.

### Claim 11 (depends on 1)

A bio-sensing device according to claim 1 wherein the electrolytic solution is a buffer having a neutral pH.

### Claim 12 (depends on 1)

A bio-sensing device according to claim 1, wherein the impedimetric analyzer comprises a counterelectrode disposed in the flow cell, and is configured to use the exposed surface on which the probe DNA and/or RNA is bound as the working electrode.

### Claim 13 (depends on 1)

A bio-sensing device according to claim 1, wherein
the impedimetric analyzer comprises a counterelectrode disposed in the flow cell, and is configured to use the exposed surface on which the probe DNA and/or RNA is bound as the working electrode;
the electrolytic solution is a buffer having a neutral pH; and
the chemical that is able to achieve denaturation of DNA and/or RNA is NaOH.

### Claim 14 (depends on 13)

A bio-sensing device according to claim 13, wherein the probe DNA and/or RNA is NH2-modified single stranded DNA covalently bound to the surface exposed to liquid flow within the flow cell through a fatty acyl group.

### Claim 15 (depends on 14)

A bio-sensing device according to claim 14, wherein the substrate is nanocrystalline diamond, or a semiconductor substrate having a diamond coating 50-150 nm in thickness at its surface exposed to liquid flow within the flow cell.