Method for treating a blood component containing sample
AssigneeARKRAY, Inc.
InventorHidenori Takagi
Life of this patent
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Abstract
Provided is a treatment method for damaging an erythrocyte and a leukocyte while suppressing damage to cells other than blood cells present in blood. In an embodiment, the disclosure relates to a method for treating a sample containing blood components, the method including mixing a sample containing blood components with a surfactant A, where the surfactant A is a nonionic surfactant represented by General formula R1—O-(EO)n-R2 (I).
Claims4
A method for separating or detecting a tumor cell and/or a cancer cell in a sample containing blood components, the method comprising treating the sample by mixing the sample with a liquid containing a surfactant A and a liquid containing a surfactant B, or with a liquid containing the surfactant A and the surfactant B,
- wherein the surfactant A is a nonionic surfactant of formula (I)
- R1—O-(EO)n—R2 (I)
- wherein:
- R1 is a hydrocarbon group having a branched chain and a carbon number in the range of 12 to 40,
- EO is an oxyethylene group,
- n is an average addition mole number of EO in the range of 23 to 50, and
- R2 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3;
- and
- the surfactant B is a nonionic surfactant selected from the group consisting of a sorbitan fatty acid ester, a compound of formula (III), a compound of formula (IV), a compound of formula (VI) and combinations thereof,
- R3—O-(AO)m/(EO)n—R4 (III)
- wherein:
- R3 is an alkyl group having a carbon number in the range of 1 to 24,
- R4 is a hydrogen atom or an alkyl group having a carbon number in the range of 1 to 3,
- AO is an oxyalkylene group having a carbon number in the range of 3 to 6,
- EO is an oxyethylene group,
- m and n are average addition mole numbers of AO and EO respectively, where m is a number in the range of 1 to 100 and n is a number in the range of 0 to 50, and
- “/” indicates that the AO group and the EO group may be added at random or as a block regardless of the order;
- R5—O-(EO)n—R6 (IV)
- wherein:
- R5 is a hydrocarbon group having a carbon number in the range of 12 to 40,
- EO is an oxyethylene group,
- n is an average addition mole number of EO in the range of 8 to 22, and
- R6 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3;
- R7—COO-(EO)n—R8 (VI)
- wherein:
- R7 is a hydrocarbon group having a carbon number in the range of 10 to 40,
- EO is an oxyethylene group,
- n is an average addition mole number of EO in the range of 6 to 160, and
- R8 is a hydrogen atom or a hydrocarbon group having a carbon number in the range of 1 to 3,
- where the surfactant B has a lytic property with respect to an erythrocyte higher than the lytic property of the surfactant A with respect to an erythrocyte, and
- wherein the method eliminates erythrocytes and leukocytes present in the sample while suppressing damage to the tumor cell and/or the cancer cell.
Dependencies are inferred from the claim text.