# US 9,429,249 B2 · Fluid triggerable valves > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,429,249 B2 - Document ID: US9429249B2 - Status: Granted - Kind: B2 - Application number: 14/465,345 - Filed: 2014-08-21 - Published: 2015-02-12 - Granted: 2016-08-30 - Earliest claimed: 2013-08-08 - Assignees: Universiteit Leiden - Applicants: Universiteit Leiden - Inventors: Paul Vulto; Sebastiaan Johannes Trietsch; Ender Yildirim - Web page: https://patentgrep.com/patent/US9429249B2 ## CPC - F16K 99/0026: {Valves using channel deformation} - B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)} - B01L 3/502746: {characterised by the means for controlling flow resistance, e.g. flow controllers, baffles or throttle valves} - B01L 2400/0406: capillary forces - B01L 2400/0688: surface tension valves, capillary stop, capillary break - F16K 2099/0084: {Chemistry or biology, e.g. "lab-on-a-chip" technology} - Y10T 137/7859: Single head, plural ports in parallel - F16K: VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING {(devices for emptying and evacuating the excess liquid in valves or conduits F16L55/07)} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION ## Abstract The present invention relates to a fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus. ## Claims ### Claim 1 A fluid triggerable passive valve for controlling flow of one or more fluids comprising: a volume comprising at least two capillary pressure barriers for respectively pinning a first and second fluid-fluid meniscus, the two capillary pressure barriers dividing the volume in at least three sub volumes, whereby two sub volumes being positioned upstream and one sub volume being positioned downstream with respect to the advancement direction of each respective fluid-fluid meniscus; wherein the capillary pressure barriers are spaced apart such that upon pinning of a first fluid-fluid meniscus on a first capillary pressure barrier and arrival or pinning of a second fluid-fluid meniscus on or at a second capillary pressure barrier, the two menisci touch and thereby coalesce into one fluid-fluid meniscus. ### Claim 2 (depends on 1) A valve according to claim 1, where upon coalescence of the two fluid-fluid menisci, the pressure required for breaching the pinned state of the resulting fluid-fluid meniscus is lower than the pressure required for breaching the pinned state of one or more fluid-fluid meniscus before coalescence. ### Claim 3 (depends on 1) A valve according to claim 1, wherein upon coalescence of the two fluid-fluid menisci the pinning of the resulting fluid-fluid meniscus is relieved and the meniscus is permitted to advances in downstream direction. ### Claim 4 (depends on 1) A valve according to claim 1, whereby at least one of each sub-volumes comprises or is a microfluidic channel. ### Claim 5 (depends on 1) A valve according to claim 1, where at least one capillary pressure barrier is stabilized by (a) subtending at both ends an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90°, and/or (b) wherein the capillary pressure is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fluid-fluid meniscus upon alignment along the capillary pressure barrier in the absence of the stretching barrier, the stretching barrier being shaped such that at least one directional component is orthogonal to the capillary pressure barrier and/or (c) the capillary pressure barrier is stabilized by subtending at one end an angle with a wall of the volume that on the downstream side of the capillary pressure barrier is greater than 90°, and at the other end is stabilized by providing a stretching barrier at a distance less than the maximum stretching distance of the fluid-fluid meniscus upon alignment along the capillary pressure barrier in the absence of the stretching barrier, the stretching barrier being shaped such that at least one directional component is orthogonal to the capillary pressure barrier. ### Claim 6 (depends on 5) A valve according to claim 5, wherein the stretching barrier is defined or includes one or more of: iv) a recess or groove defined in the material of a wall of the volume; v) a protuberance from a wall of the volume into the volume; vi) a bend or recess opening into a further channel or reservoir; vii) a line defined in or on the material of a wall of the volume that is of lower wettability than the material of the said wall adjacent the line. ### Claim 7 (depends on 5) A valve according claim 5, wherein the two capillary pressure barriers are stabilized by at least one common stretching barrier. ### Claim 8 (depends on 7) A valve according to claim 7, whereby coalescence of the two menisci results in breaching the at least one common stretching barrier. ### Claim 9 (depends on 1) A valve according to claim 1, wherein the capillary pressure barrier is defined by or includes one or more of: i) a recess or groove defined in the material of a wall of the volume; ii) a protuberance from a wall of the volume into the volume; and/or iii) a line defined in or on the material of a wall of the volume that is of lower wettability than the material of the said wall adjacent the line. ### Claim 10 (depends on 1) A valve according to claim 1, wherein the distance that the two capillary pressure barriers are spaced apart is less than twice the maximum stretching distance of each fluid-fluid meniscus that is pinned on one said capillary pressure barrier in the absence of the second fluid-fluid meniscus. ### Claim 11 (depends on 10) A valve according to claim 10, wherein the maximum stretching distance, ds, is defined by formula I: d s = g ⁡ ( cos ⁢ ⁢ θ 2 - sin ⁢ ⁢ θ 1 cos ⁢ ⁢ θ 1 - sin ⁢ ⁢ θ 2 ) , ( I ) wherein g represents the distance between the first substrate on which the first capillary pressure barrier is provided and the second substrate facing the substrate on which the first capillary pressure barrier is provided; wherein θ1 represents the contact angle of the fluid with the material of the substrate facing the first capillary pressure barrier; and wherein θ2 represents the contact angle of the fluid with the capillary pressure barrier material. ### Claim 12 (depends on 1) A valve according to claim 1, wherein the volume comprises two capillary pressure barriers for holding two fluid-fluid menisci and an intermediate volume defined by the two capillary pressure barriers and the menisci once pinned, wherein the two capillary pressure barriers converge at a minimal distance at least at one point thereby reducing the intermediate volume for allowing the menisci to contact at this point upon pinning of both menisci and/or stretching of at least one meniscus. ### Claim 13 (depends on 1) A valve according to claim 1, further comprising means for applying a pressure on at least one of the fluid streams to stretch one or more of the menisci. ### Claim 14 (depends on 13) A valve according to claim 13, further comprising at least one upstream channel and at least one downstream channel provided in fluid connection to the volume. ### Claim 15 (depends on 1) A microfluidic circuit including at least one microfluidic chamber and at least one fluid triggerable valve according to claim 1. ### Claim 16 (depends on 15) A microfluidic circuit according to claim 15, comprising at least two fluid triggerable valves, wherein the first valve requires a different bursting pressure with respect to the second valve, thereby allowing a selective operation of valves. ### Claim 17 (depends on 15) A microfluidic circuit according to claim 15, whereby the distance between the two capillary pressure barriers contained by the first valve differs from the distance between the two capillary pressure barriers contained by at least a second valve. ### Claim 18 (depends on 15) A microfluidic circuit according to claim 15, whereby the distance between the at least one capillary pressure barrier and at least one stretching barrier contained by the first valve differs from the distance between the at least one capillary pressure barrier and at least one stretching barrier contained by the second said valve. ### Claim 19 (depends on 1) Use of a valve according to claim 1 for the control of microfluidic fluid flows. ### Claim 20 (depends on 1) A process for the controlled release of a microfluidic flow, comprising providing at least a first and a second fluid stream to a fluid triggerable valve or circuit according to claim 1, and increasing the pressure until the first and second fluid stream merge through operation of the valve.