# US 9,428,808 B2 · Markers, biochips and kits for milk quality detection

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- Number: US 9,428,808 B2
- Document ID: US9428808B2
- Status: Granted
- Kind: B2
- Application number: 13/509,433
- Filed: 2010-05-27
- Published: 2012-10-04
- Granted: 2016-08-30
- Earliest claimed: 2009-11-13
- Assignees: Jiangsu Micromedmark Biotech Co., Ltd.
- Applicants: Chengyu Zhang; Ke Zeng; Junfeng Zhang; Chunliang Tian; Haijin Li
- Inventors: Chengyu Zhang; Ke Zeng; Junfeng Zhang; Chunliang Tian; Haijin Li
- Web page: https://patentgrep.com/patent/US9428808B2

## CPC

- C12Q 1/6876: Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q 2600/178: miRNA, siRNA or ncRNA
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

This invention provides markers, methods, biochips and kits for milk quality detection. The present invention particularly provides a method for milk quality detection by means of detecting the particular microRNAs in milk, so as to establish the standard of raw milk content.

## Claims

### Claim 1

A biochip which is a solid carrier that has attached thereto a set of probes wherein the set of probes consists of oligonucleotides comprising each of SEQ ID NO: 21-117.

### Claim 2

A set of labeled probes consisting of oligonucleotides comprising each of SEQ ID NO: 21-117, wherein the probes are labeled with an isotope label, a biotin label, or a fluorescence label.

### Claim 3 (depends on 1, 2)

A composition consisting of the biochip of claim 1 or the set of labeled probes of claim 2 combined with isolated RNA from a bovine cow milk product.

### Claim 4 (depends on 1, 2)

A kit consisting of the biochip of claim 1 or the set of labeled probes of claim 2 and additionally primers for amplifying target miRNA sequences to hybridize to the probes on the biochip of claim 1 or in the set of labeled probes of claim 2.