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US 9,428,794 B2Granted
Methods of depleting a target nucleic acid in a sample and kits for practicing the same
AssigneeTakara Bio USA, Inc.
InventorsAndrew Farmer, Ian Oglesby, Magnolia Bostick, Steve Oh
Life of this patent
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Abstract
Provided are methods of depleting a target nucleic acid in a sample. The methods include contacting a target nucleic acid with two or more polymers that specifically hybridize to the target nucleic acid, and cleaving the hybridized regions of the target nucleic acid to deplete the target nucleic acid in the sample. Kits for practicing the subject methods are also provided.
Claims22
A method of depleting an undesired target ribosomal ribonucleic acid (rRNA) in a sample and enriching for a desired RNA, the method comprising:
- obtaining a total RNA sample comprising a desired RNA and an undesired target rRNA;
- contacting the sample with a polymer cocktail comprising at least two synthetic oligonucleotides selected from SEQ ID NOs:06-177 that specifically hybridize to distinct regions of an undesired 18S target rRNA or an undesired 28S target rRNA in the sample to generate hybridized regions of the undesired target rRNA that are separated by a gap;
- cleaving the hybridized regions to deplete the undesired target rRNA in the sample;
- and
- collecting the desired RNA or removing the cleaved undesired target rRNA to enrich the for the desired RNA.
2 more elements
Dependencies are inferred from the claim text.