DNA polymerases with improved activity
AssigneeRoche Molecular Systems, Inc.
InventorsKeith Bauer, Thomas W. Myers, Shawn Suko
Life of this patent
Estimating the term…
Abstract
Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
Claims12
A method of reverse transcribing an RNA target comprising reverse transcribing cDNA from an RNA template with a DNA polymerase comprising an amino acid sequence at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid of the DNA polymerase corresponding to position 640 of SEQ ID NO:1 is any amino acid other than I.
Dependencies are inferred from the claim text.