Oscillating amplification reaction for nucleic acids
AssigneeMesa Biotech, Inc.
InventorsHong Cai, Nathan J. Cobb
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Abstract
One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.
Claims49
A method for amplifying a template of nucleic acid target sequence contained in a sample comprising:
- contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence;
- oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein a change in temperature is no greater than 20° C. during a plurality of temperature cycles;
- and
- amplifying the template of nucleic acid target sequence.
A method for amplifying a template of nucleic acid target sequence contained in a sample comprising:
- contacting the sample with an amplification reaction mixture comprising:
- a primer or a primer pair having a length of between 35-70 base pairs and complementary to the template of the nucleic acid target sequence and wherein the melting temperature of each primer of the primer pair is between 70-80° C.;
- DMSO;
- monovalent cation;
- divalent cation;
- dNTPs;
- and
- DNA Polymerase;
- oscillating a temperature of the reaction between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles;
- and
- amplifying the template of nucleic acid target sequence.
Dependencies are inferred from the claim text.