Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch sustrates

A process for producing residual starch from a granular starch slurry, said process comprising:

• contacting a granular starch slurry obtained from a granular starch substrate simultaneously with an alpha amylase and a glucoamylase, wherein the glucoamylase has at least 97% amino acid sequence identity with SEQ ID NO:
• 3, at a temperature equal to or below the gelatinization temperature of the granular starch;
• allowing the alpha amylase and the glucoamylase to act for a period of time sufficient to hydrolyze the granular starch to obtain glucose and residual starch;
• and,
• retaining the residual starch to produce residual starch from the granular starch slurry.