Transformation of algae for increasing lipid production
AssigneeSapphire Energy, Inc.
InventorsChristopher Yohn, April Brand, Michael Mendez, Craig A. Behnke
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Abstract
The present disclosure provides novel proteins that when over expressed in algae result in an increase or change in fatty acid and/or glycerol lipid production and/or accumulation, without a substantial decrease in the growth rate of the alga or the break down of algal components, such as chlorophyll. The present disclosure also describes methods of using the novel proteins to increase or change the production and/or accumulation of fatty acids and/or glycerol lipids in algae. In addition, these proteins are useful tools in obtaining information about the fatty acid and triacyglyceride (TAG) synthetic pathways in algae.
Claims25
A non-vascular photosynthetic organism transformed with a polynucleotide, wherein the polynucleotide comprises:
- (a) a nucleic acid sequence of SEQ ID NO:
- 9, SEQ ID NO:
- 12, or SEQ ID NO:
- 4;
- or (b) a nucleic acid sequence with at least 95%, at least 98%, or at least 99% sequence identity to the nucleic acid sequence of SEQ ID NO:
- 9, SEQ ID NO:
- 12, or SEQ ID NO:
- 4, wherein the transformed organism has an increased lipid content than the untransformed organism.
A method of increasing lipid content of a non-vascular photosynthetic organism, comprising:
- i) transforming the organism with a polynucleotide comprising a nucleotide sequence encoding a protein expressed in the transformed organism, wherein expression of the protein results in an increase in the lipid content of the transformed organism as compared to the untransformed organism, and wherein the nucleotide sequence comprises, (a) a nucleic acid sequence of SEQ ID NO:
- 9, SEQ ID NO:
- 12, or SEQ ID NO:
- 4, or (b) a nucleic acid sequence with at least 95%, at least 98%, or at least 99% sequence identity to the nucleic acid sequence of SEQ ID NO:
- 9, SEQ ID NO:
- 12, or SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.