Transformant and method for producing same, and method for producing lactic acid
AssigneeAsahi Glass Company, Limited
InventorsFutoshi Hara, Shuichiro Kimura
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Abstract
Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.
Claims13
A Schizosaccharomyces pombe transformant, wherein the transformant is transformed with a Lactobacillus pentosus lactate dehydrogenase gene and a human lactate dehydrogenase gene, and wherein a chromosomal pyruvate decarboxylase (PDC) gene in the transformant is deleted or inactivated, wherein the Lactobacillus pentosus lactate dehydrogenase gene and the human lactate dehydrogenase gene are expressed in the transformant, and wherein the transformant produces lactic acid.
Dependencies are inferred from the claim text.