Methods of producing acetoin and 2,3-butanediol using photosynthetic microorganisms
AssigneeThe Regents of the University of California
InventorsShota Atsumi, John W. K. Oliver, Iara M. P. Machado
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Abstract
The present disclosure provides recombinant cyanobacteria having acetolactate synthase activity and acetolactate decarboxylase activity, as well as recombinant cyanobacteria having acetolactate synthase activity, acetolactate decarboxylase activity, and secondary alcohol dehydrogenase activity. Methods for the production of the recombinant cyanobacteria, as well as methods for use thereof for production of acetoin and 2,3-butanediol from carbon dioxide and light are also provided.
Claims20
A cyanobacterium comprising a recombinant polynucleotide encoding an acetolactate synthase (ALS) and an acetolactate decarboxylase (ALDC), wherein the ALS is a Bacillus sp. ALS and the ALDC is an Enterobacter sp. ALDC or an Aeromonas sp. ALDC, and wherein expression of the ALS and the ALDC results in an increase in production of acetoin as compared to a corresponding cyanobacterium lacking the polynucleotide.
A cyanobacterium comprising a recombinant polynucleotide encoding an acetolactate synthase (ALS), an acetolactate decarboxylase (ALDC) and a secondary alcohol dehydrogenase (sADH), wherein the sADH is selected from the group consisting of a Candida sp. sADH, a Leuconostoc sp. sADH, a Clostridium sp. sADH, and a Thermoanaerobacter sp. sADH, and wherein expression of the ALS, the ALDC and the sADH results in an increase in production of one or both of acetoin and 2,3-butanediol (23BD) as compared to a corresponding cyanobacterium lacking the polynucleotide.
Dependencies are inferred from the claim text.