Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
AssigneeMonsanto Technology LLC
InventorStanislaw Flasinski
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Abstract
The materials and methods disclosed provide for polynucleotide molecules sufficiently divergent from polynucleotides naturally contained in plants, or polynucleotides previously introduced into plants as transgenes to permit trait stacking in plant breeding methods or plant transformation methods. The disclosure also provides for methods and compositions to detect the polynucleotides of the invention in plants.
Claims10
A method to avoid silencing of a transgene comprising SEQ ID NO:16 operably linked to a polynucleotide encoding a chloroplast transit peptide in transgenic plants comprising the steps of:
- (a) obtaining a DNA construct comprising an artificial polynucleotide molecule comprising a nucleic acid encoding the EPSPS polypeptide encoded by SEQ ID NO:35 operably linked to a polynucleotide encoding a chloroplast transit peptide;
- (b) transforming a plant cell with said DNA construct;
- and
- (c) regenerating said plant cell into a fertile transgenic plant,
- wherein said fertile transgenic plant comprises both said artificial polynucleotide and said transgene.
An artificial polynucleotide molecule comprising SEQ ID NO:35.
A plant cell comprising at least a first and a second polynucleotide, wherein the first polynucleotide comprises SEQ ID NO:35, and wherein the second polynucleotide comprises SEQ ID NO:16 operably linked to a polynucleotide encoding a chloroplast transit peptide.
A plant cell, seed, plant or progeny thereof comprising SEQ ID NO:35.
Dependencies are inferred from the claim text.