Nucleic acid construct with gene encoding an arabinofuranosidase polypeptide and methods of using same
AssigneeNovozymes A/S
InventorsHanne Risbjerg Soerensen, Christel Thea Joergensen, Lars Hylling Christensen, Christian Isak Joergensen, Carsten Hoerslev Hansen, Lene Venke Kofod
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Abstract
The present invention relates to isolated polypeptides having alpha-L-arabinofuranosidase activity and isolated nucleic acid sequences encoding the polypeptides, such that the nucleic acid is operably linked to a heterologous control sequence for expression. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides set forth in SEQ ID NO: 2 and fragments of SEQ ID NO: 2.
Claims13
A nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide having arabinofuranosidase activity, wherein the nucleic acid sequence is operably linked to one or more heterologous control sequences capable of directing the expression of the polypeptide in a suitable expression host, and wherein the polypeptide is selected from the group consisting of:
- (a) a polypeptide with at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the full complement of the nucleic acid sequence of nucleotides 55-1677 of SEQ ID NO:
- 1;
- wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for high stringency, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.
A recombinant host cell transformed with a nucleic acid sequence encoding a polypeptide having arabinofuranosidase activity, wherein the nucleic acid sequence is operably linked to one or more heterologous control sequences and wherein the polypeptide is selected from the group consisting of:
- (a) a polypeptide with at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- and
- (b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the full complement of the nucleic acid sequence of nucleotides 55-1677 of SEQ ID NO:
- 1;
- wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/mL sheared and denatured salmon sperm DNA, and 50% formamide for high stringency, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.
Dependencies are inferred from the claim text.