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AssigneeNovartis Vaccines and Diagnostics, Inc.
InventorsDoris Coit, Michael Houghton, Colin McCoin, Angelica Medina-Selby, Michael Vajdy
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Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described. The immunogenic compositions of the invention may also contain antigens other than Norovirus or Sapovirus antigens, including antigens that can be used in immunization against pathogens that cause diarrheal diseases, such as antigens derived from rotavirus.
A multiple epitope fusion protein comprising two or more epitopes from two or more isolates of one or more viruses selected from the group consisting of a Norovirus and a Sapovirus, wherein the two or more epitopes are encoded by one or more open reading frames selected from the group consisting of a ORF1, ORF2, and ORF3 of one or more viruses selected from the group consisting of Norovirus and Sapovirus isolate, wherein one or more of the epitopes are encoded by ORF1 of one or more viruses selected from the group consisting of Norovirus and-Sapovirus isolate.
A Norovirus multiple epitope fusion protein comprising a Norovirus N-terminal protein, a Norovirus NTPase, and a Norovirus polymerase, wherein the fusion protein does not include a full-length Norovirus polyprotein wherein the fusion protein comprises at least one heterologous linker amino acid sequence between two epitopes in the multiple epitope fusion protein.
A multiple epitope fusion protein comprising two or more epitopes from two or more isolates of one or more viruses selected from the group consisting of a Norovirus and a Sapovirus, wherein the fusion protein comprises one heterologous linker amino acid sequence between each epitope in the multiple epitope fusion protein.
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