Vesicular stomatitis viruses
AssigneeMayo Foundation for Medical Education and Research
InventorsStephen James Russell, Shruthi Naik
Life of this patent
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Abstract
This document provides methods and materials related to vesicular stomatitis viruses. For example, vesicular stomatitis viruses, nucleic acid molecules encoding VSV polypeptides, methods for making vesicular stomatitis viruses, and methods for using vesicular stomatitis viruses to treat cancer are provided.
Claims13
A vesicular stomatitis virus comprising an RNA molecule, wherein said RNA molecule comprises, in a 3′ to 5′ direction, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV N polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV P polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV M polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding an interferon (IFN) polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV G polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a Sodium Iodide Symporter (NIS) polypeptide, and a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV L polypeptide, wherein said positive sense transcript encoding said IFN polypeptide comprises CUAACAG followed by a sequence encoding said IFN polypeptide followed by UAUGAAAAAAA (RNA of SEQ ID NO:1), and wherein said positive sense transcript encoding said NIS polypeptide comprises CUAACAG followed by a sequence encoding said NIS polypeptide followed by UAUGAAAAAAA (RNA of SEQ ID NO:1).
A composition comprising a vesicular stomatitis virus comprising RNA molecule, wherein said RNA molecule comprises, in a 3′ to 5′ direction, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV N polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV P polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV M polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding an IFN polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV G polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a NIS polypeptide, and a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV L polypeptide, wherein said positive sense transcript encoding said IFN polypeptide comprises CUAACAG followed by a sequence encoding said IFN polypeptide followed by UAUGAAAAAAA (RNA of SEQ ID NO:1), and wherein said positive sense transcript encoding said NIS polypeptide comprises CUAACAG followed by a sequence encoding said NIS polypeptide followed by UAUGAAAAAAA (RNA of SEQ ID NO:1).
A nucleic acid molecule comprising a nucleic acid strand comprising, in a 3′ to 5′ direction, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV N polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV P polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV M polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding an IFN polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV G polypeptide, a nucleic acid sequence that is a template for a positive sense transcript encoding a NIS polypeptide, and a nucleic acid sequence that is a template for a positive sense transcript encoding a VSV L polypeptide, wherein said positive sense transcript encoding said IFN polypeptide comprises CUAACAG followed by a sequence encoding said IFN polypeptide followed by UAUGAAAAAAA (RNA of SEQ ID NO:1), and wherein said positive sense transcript encoding said NIS polypeptide comprises CUAACAG followed by a sequence encoding said NIS polypeptide followed by UAUGAAAAAAA (RNA of SEQ ID NO:1).
Dependencies are inferred from the claim text.