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US 9,428,732 B2Granted
Compositions and methods for differentiating pluripotent stem cells into primitive blood cells and uses thereof
AssigneePrimorigen Biosciences, LLC
InventorsNiels-Bjarne Woods, Roger Ronn, Carolina Guibentif
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Abstract
Compositions and methods that employ various combinations of such factors as retinoic acid signaling inhibitors, antioxidants, BMP4, VEGF, prostaglandin E2 pathway stimulants, TPO, SCF, FLT-3, EPO, TGFβ1, p38 MAPK inhibitors, beta adrenergic receptor agonists, cell cycle inhibitors, RXR agonists, Cripto, and chromatin remodelers to drive differentiation of pluripotent stem cells towards primitive blood cells. Uses of such primitive blood cells are provided.
Claims19
A differentiation medium comprising:
- a retinoic acid signaling inhibitor comprising DEAB;
- an antioxidant comprising ascorbic acid;
- a stimulant of prostaglandin E2 pathway comprising prostaglandin E2 (PGE2);
- a P38 MAPK inhibitor comprising LY2228820;
- BMP4;
- vascular endothelial growth factor (VEGF);
- and
- a beta adrenergic receptor agonist comprising norepinephrine.
5 more elements
Dependencies are inferred from the claim text.