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US 9,428,729 B2Granted
Method of reseeding adherent cells grown in a hollow fiber bioreactor system
AssigneeTerumo BCT, Inc.
InventorsGlen Delbert Antwiler, David A. Windmiller, Monique Givens
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Abstract
This invention is directed to methods of directly reseeding harvested adherent cells grown in a hollow fiber bioreactor. Also disclosed is a novel harvest media for use in directly reseeding adherent cells into a hollow fiber bioreactor.
Claims4
A media for harvesting cells grown in a hollow fiber bioreactor, the media comprising:
- base media;
- protein;
- 3.9 mM calcium chloride;
- 9.91 mM anhydrous D-glucose;
- and
- 2.24 mM magnesium sulfate.
3 more elements
Dependencies are inferred from the claim text.