Carrier for undifferentiated cell culture and subculture method thereof
AssigneeCoorsTek, Inc.
InventorsFumihiko Kitagawa, Takafumi Imaizumi, Katsunori Sasaki
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Abstract
A colony (cell mass) proliferated under the undifferentiated state is obtained by using a carrier for cell culture in which two or more of a concavity having a porous body in a surface are arranged on a substrate surface in the form of a matrix, inoculating an undifferentiated cell on at least one concavity of the carrier for culture and carrying out culture.
Claims10
A carrier for cell culture, comprising concavities arranged in line direction and column direction on a surface of the carrier, wherein the whole carrier, including the surface, consists of a porous body and wherein a cultured cell is capable of adhering to the inside of each of the concavities, and wherein the porous body has a pore size of from 10 nm to 2,000 nm, and wherein the porous body consists of at least one material selected from the group consisting of zirconia, yttria, titania, alumina, hydroxyapatite, and β-calcium tertiary phosphate, and
- wherein the concavities each have a diameter from 10 μm to 1,000 μm and a depth of from 30 μm to 1,000 μm.
Dependencies are inferred from the claim text.