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US 9,428,726 B2Granted
Modified host cells with efflux pumps
AssigneeThe Regents of the University of California
InventorsMary J. Dunlop, Jay D. Keasling, Aindrila Mukhopadhyay
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Abstract
The present invention provides for a modified host cell comprising a heterologous expression of an efflux pump capable of transporting an organic molecule out of the host cell wherein the organic molecule at a sufficiently high concentration reduces the growth rate of or is lethal to the host cell.
Claims18
A modified host cell comprising:
- (a) an efflux pump comprising an amino sequence having at least 95% identity as compared to the amino acid sequence of Azotobacter vinelandii AvOP MexEF, Pseudomonas putida KT2440 TtgABC, Pseudomonas putida KT2440 MexEF-OprN, Pseudomonas aeruginosa PA01 MexAB-OprM, Pseudomonas aeruginosa PA01 MexEF-OprN, Escherichia coli K12 AcrAB, Pseudomonas putida KT2440 NP_745594, Pseudomonas aeruginosa PA01 NP_250708, Marinobacter aqueolei YP_960752, Marinobacter aqueolei YP_957870, or Alcanivorax borkumensis SK2 YP_692684, wherein said efflux pump is heterologous to the modified host cell, and capable of transportation of an organic molecule out of the modified host cell, and (b) a recombinant nucleic acid encoding one or more enzymes for producing the organic molecule;
- wherein the organic molecule at a sufficiently high concentration inhibits or reduces the growth rate of or is lethal to the modified host cell, the modified host cell is a bacterium and the organic molecule is a C4-C12 alcohol, pinene, geranyl acetate, geraniol, limoene, or faresyl hexanoate.
Dependencies are inferred from the claim text.