Bioreactor with feed and harvest flow through filter assembly
AssigneeGE Healthcare Bio-Sciences AB
InventorPatric Fricking
Life of this patent
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Abstract
The present invention relates to a bioreactor and a method for operating a bioreactor which comprises a chamber (100) capable of receiving a liquid media, and a filter assembly (200) comprising a perfusion filter (210; 212; 214). The filter assembly (200) is disposed in the chamber (100) and the filter assembly (200) is free to move within the chamber (100). The filter assembly (200) comprises means for coupling a harvesting flow through said filter (210; 212; 214) and means for coupling a feed flow through said filter in an opposite direction compared to the harvesting flow. Through this arrangement, the clogging prevention of the perfusion filter can be improved.
Claims10
A bioreactor comprising:
- a chamber capable of receiving a liquid media;
- and
- a filter assembly disposed in and free to move about the chamber;
- wherein the filter assembly is configured to:
- (i) receive a feed flow through a feed flow tube passing through a pathway formed between a first perfusion filter and a second perfusion filter of the filter assembly, the feed flow being pumped into the bioreactor by means of a feed pump;
- and
- (ii) produce a harvesting flow through the first and second perfusion filters and through a filtration tube in an opposite direction of the feed flow, the harvesting flow being pumped from the bioreactor by means of a harvest pump.
A method of operating a bioreactor including a chamber capable of receiving a liquid media and a filter assembly disposed in and free to move about the chamber, the method comprising:
- receiving a feed flow through a feed flow tube passing through a pathway formed between a first perfusion filter and a second perfusion filter of the filter assembly, the feed flow being pumped into the bioreactor by means of a feed pump;
- and
- producing a harvesting flow through the first and second perfusion filters and through a filtration tube in an opposite direction of the feed flow, the harvesting flow being pumped from the bioreactor by means of a harvest pump.
Dependencies are inferred from the claim text.