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US 9,428,723 B2Granted
Micro-incubation systems for microfluidic cell culture and methods
AssigneeEMD Millipore Corporation
InventorsPhilip J. Lee, Terry Gaige, Wei Hsuan (Jessie) Ho
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Abstract
A micro-incubator manifold for improved microfluidic configurations and systems and methods of manufacture and operation for a manifold and automated microfluidic systems.
Claims14
A microincubator manifold, comprising:
- a top layer carrying one or more pneumatic channels for conveying pressure or gas to a culture plate;
- a bottom layer comprising a gasket for providing a removable air-tight seal to the culture plate;
- and a heat exchange module, attached to the top layer;
- wherein the manifold seals to the culture plate thereby enclosing an incubation volume of gas;
- the incubation volume of gas circulating between a culture chamber or observation area of the culture plate and a heat exchange area of the heat exchange module;
- thereby providing a controlled temperature and gas composition above and in communication with the culture chamber or observation area.
3 more elements
Dependencies are inferred from the claim text.